콩 조직배양 기술에 기반한 생명공학 연구 동향 = Status of Molecular Biotechnology Research Based on Tissue Culture of Soybean

다국어 초록 (Multilingual Abstract)

Soybean (Glycine max (L.) Merrill) is one of the most important crops of the world. With the completion of the soybean genome sequence, the Korean soybean core collection consisted of 430 accessions with genetic and phenotypic diversity was constructed in recent year. The availability of genome sequences and core collection will result in the crop improvement by molecular breeding using the various accessions and genome editing approaches. Efficient tissue culture techniques, such as haploid production, protoplast culture and plant regeneration from various organs are essential for the successful molecular biological approach and crop improvement. However, soybean is still considered to be recalcitrant in tissue culture because of the low frequency of regeneration and limitation of available responsive cultivars. In this study, we discuss the recent studies of tissue culture technology and methodology for efficient tissue culture to genetic improvement and application of molecular biotechnology in soybean.

국문 초록 (Abstract)

콩은 전세계적으로 재배되는 중요한 작물 중에 하나로 최근, 표준유전체 해독과 함께 유전적, 표현형적으로 다양성을 가진한국핵심집단이 구축됨에 따라 유전체 기반 분자 육종 연구, 유전자 교정 기술을 활용한 새로운 육종 소재 개발 연구가 가속화될 것으로 예상된다. 유전체 정보 기반 작물의 분자 육종 및 생명공학 연구를 통한 성공적인 작물의 개량을 위해서는 식물의효율적인 조직배양 기술이 수반되어야 할 것이다. 그러나 반수체 생산, 원형질체 배양 및 형질전환 기술과 같은 콩의 조직배양효율은 아직까지 높지 않고 일부 계통에 한정되어 이루어지고있다. 본 논문에서는 콩의 분자육종 및 생명공학 기술의 적용을위하여 다양한 콩 조직배양 기술에 관한 연구 동향을 분석하고조직배양 효율에 영향을 미치는 요인들에 대한 정보를 제공하고자 하였다.

참고문헌 (Reference)

1 전재범, "변이밀집영역 유래 27개 InDel 마커를 이용한 콩(Glycine max (L.) Merrill) 신품종 판별 및 국내 149 품종과 유연관계 분석" 한국자원식물학회 32 (32): 519-542, 2019

2 서미숙, "배추 유전자원의 소포자 유래 배 발생 효율에 미치는 배양 조건 구명" 한국식물생명공학회 41 (41): 116-122, 2014

3 서미숙, "기내배양을 통한 Brassica 작물의 반수체 생산에 관한 연구 동향" 한국국제농업개발학회 27 (27): 522-528, 2015

4 김동건, "기내 배양을 통한 국내 콩(Glycine max L.) 품종의 식물체 재분화" 한국자원식물학회 29 (29): 143-153, 2016

5 Li, R., "Vernonia DGATs increase accumulation of epoxy fatty acids in oil" 8 : 184-195, 2010

6 Vasil, I. K., "Transformation of wheat via particle bombardment" 318 : 273-283, 2006

7 McCabe, D. E., "Transformation of elite cotton cultivars via particle bombardment of meristems" 11 : 596-598, 1993

8 Blanca, J., "Transcriptome sequencing for SNP discovery across Cucumis melo" 13 : 280-, 2012

9 Gazzarrini, S., "The transcription factor FUSCA3 controls development timing in Arabidopsis through the hormones gibberellin and abscisic acid" 7 : 373-385, 2004

10 Zieg, R. G., "The isolation, culture and callus formation of soybean pod protoplasts" 18 (18): 105-114, 1980

11 Bolibok, H., "The identification of QTLs associated with the in vitro response of rye (Secale cereal L.)" 12 : 523-535, 2007

12 Velasco, R., "The genome of the domesticated apple (Malus × domestica Borkh.)" 42 : 833-839, 2010

13 Thakare, D., "The MADS-domain transcriptional regulator AGAMOUS-LIKE15promotes somatic embryo development in Arabidopsis and soybean" 146 (146): 1663-1672, 2008

14 Valente, M. A. S., "The ER luminal binding protein (BiP)mediates an increase in drought tolerance in soybean and delays drought-induced leaf senescence in soybean and tobacco" 60 : 533-546, 2009

15 Hecht, V., "The Arabidopsis somatic embryogenesis receptor kinase 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture" 127 : 803-816, 2001

16 Sun, X., "Targeted mutagenesis in soybean using the CRISPRCas9system" 5 : 10342-, 2015

17 Guo, W. W., "Targeted cybridization in citrus: transfer of Satuma cytoplasm to seedy cultivars for potential seedlessness" 22 : 752-758, 2004

18 Touraev, A., "Stress-induced microspore embryogenesis in tobacco : An optimized system for molecular studies" 15 : 561-565, 1996

19 Shetty, K., "Stimulation of in vitro shoot organogenesis in Glycine max (Merrill.) by allantoin and amides" 81 : 245-251, 1992

20 Lin, W., "Soybean protoplast culture and direct gene uptake and expression by cultured soybean protoplasts" 84 : 856-861, 1987

21 Singh, R. J., "Soybean genetic resources and crop improvement" 42 : 605-616, 1999

22 Tougou, M., "Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene" 26 : 1967-1975, 2007

23 Trick, H. N., "Sonication-assisted Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill] embryogenic suspension culture tissue" 17 : 482-488, 1998

24 Collonnier, U., "Somatic hybrids between Solanum melongena and S. sisymbrifolium, as a useful source of resistance against bacterial and fungal wilts" 164 : 849-861, 2003

25 Melchers, G., "Somatic hybridisation of plants by fusion of protoplasts" 135 (135): 277-294, 1974

26 Gamborg, O. L., "Somatic embryogenesis in cell cultures of Glycine species" 2 : 209-212, 1983

27 Karthik, S., "Sodium nitroprusside enhances regeneration and alleviates salinity stress in soybean [Glycine max (L.)Merrill]" 19 : 101173-, 2019

28 Flores, T., "Silencing of GmFAD3gene by siRNA leads to low α-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]" 17 : 839-850, 2008

29 Aragão, F. J. L., "Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merrill] plants at a high frequency" 101 : 1-6, 2000

30 Barwale, U. B., "Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node" 72 : 423-428, 1986

31 Wang, X., "Research progression soybean tissue culture system and transformation" 28 : 731-735, 2009

32 Dhir, S. K., "Regeneration of fertile plants from protoplasts of soybean (Glycine max L. Merr.): Genotypic differences in culture response" 11 (11): 285-289, 1992

33 Zeng, P., "Refined glufosinate selection in Agrobacterium-mediated transformation of soybean [Glycine max (L.) Merrill]" 22 : 478-482, 2004

34 Parrott, W. A., "Recovery and evaluation of soybean plants transgenic for a bacillus thuringiensis var. kurstaki insecticidal gene" 30 : 144-149, 1994

35 Hansen, G., "Recent advances in the transformation of plants" 4 (4): 226-231, 1999

36 Newell, C. A., "Protoplast culture and plant regeneration in Glycine canescens F" 4 (4): 145-149, 1985

37 Sato, S., "Production of γ-linolenic acid and stearidonic acid in seeds of marker-free transgenic soybean" 44 : 646-652, 2004

38 Hinchee, M., "Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer" 6 : 915-922, 1988

39 Keller, W. A., "Production of haploids via anther culture in Brasica oleracea var" 32 : 151-159, 1983

40 Cistué, L., "Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture" 25 : 257-264, 2006

41 Kakkar, R. K., "Polyamines and plant morphogenesis" 43 : 1-11, 2000

42 Dhir, S. K., "Plantlet regeneration from immature cotyledon protoplasts of soybean (Glycine max L.)" 10 (10): 39-43, 1991

43 Wei, Z. M., "Plant regeneration from protoplasts of soybean(Glycine max L" 7 (7): 348-351, 1988

44 Chen, L. P., "Plant regeneration from hypocotyl protoplasts of red cabbage (Brassica oleracea) by using nurse cultures" 77 : 133-138, 2004

45 Wright, M. S., "Plant regeneration by organogenesis in Glycine max" 5 (5): 150-154, 1986

46 Kao, K. N., "Plant protoplast fusion and growth of intergeneric hybrid cells" 120 (120): 215-227, 1974

47 김혜정, "Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean" 한국육종학회 5 (5): 237-242, 2017

48 Dan, Y., "Organogenic regeneration of soybean from hypocotyl explants" 34 : 14-21, 1998

49 Khalafalla, M. M., "Optimization of particle bombardment conditions by monitoring of transient sGFP (S65T) expression in transformed soybean" 55 : 257-263, 2005

50 Li, S., "Optimization of Agrobacterium-mediated transformation in soybean" 24 (24): 246-, 2017

51 Nguyen, K. L., "Next-generation sequencing accelerates crop gene discovery" 24 (24): 263-274, 2019

52 Zernova, O. V., "Modification of isoflavones in soybean seeds via expression of multiple phenolic biosynthetic genes" 47 : 769-777, 2009

53 Wheatley, W. G., "Microspore growth and anther staging in wheat anther culture" 5 : 47-49, 1986

54 Zhang, Y. X., "Microspore embryogenesis induced by low gamma dose irradiation in apple" 108 : 173-176, 1992

55 Jacquard, C., "Microspore embryogenesis and programmed cell death in barley: effects of copper on albinism in recalcitrant cultivars" 28 : 1329-1339, 2009

56 Yu, O., "Metabolic engineering to increase isoflavone biosynthesis in soybean seed" 63 : 753-763, 2003

57 Seo, M. S., "Mapping quantitative trait loci for tissue culture response in VCS3MDH population of Brassica rapa" 32 : 1251-1261, 2013

58 Taguchi-Shiobara, F., "Mapping quantitative trait loci associated with regeneration ability of seed callus in rice, Oryza sativa L" 5 : 828-833, 1997

59 Taguchi-Shiobara, F., "Mapping QTLs that control the performance of rice tissue culture and evaluation of derived near-isogenic lines" 112 : 968-976, 2006

60 Chao Yang, "Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)" 한국분자세포생물학회 32 (32): 337-342, 2011

61 Takagi, K., "Manipulation of saponin biosynthesis by RNA interference-mediated silencing of β-amyrin synthase gene expression in soybean" 30 : 1835-1846, 2011

62 Gaj, M. D., "LEAFT COTYLEDON genes are essential for induction of somatic embryogenesis of Arabidopsis" 222 : 977-988, 2005

63 Jeong, N., "Korean soybean core collection:Genotypic and phenotypic diversity population structure and genome-wide association study" 14 (14): e0224074-, 2019

64 Nishimura, A., "Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems" 102 (102): 11940-11944, 2005

65 Hammatt, A., "Isolation and culture of soybean hypocotyl protoplasts" 24 (24): 601-604, 1988

66 Bajaj, Y. P. S., "Interspecific hybridization of Brassica napus and B. juncea through ovary, ovule and embryo culture" 35 : 103-109, 1986

67 Vagadia, B. H., "Inactivation methods of soybean trypsin inhibitor-a review" 64 : 115-125, 2017

68 Raza, G., "In vitro plant regeneration from commercial cultivars of soybean" 2017 : 7379693-, 2017

69 He, G. Y., "Improvement of somatic embryogenesis and plant regeneration from durum wheat (Triticum turgidum var. durum Desf.) scutellum and inflorescence cultures" 119 : 369-376, 2001

70 Mano, Y., "Identification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.)" 105 : 708-715, 2002

71 Matzke, A. J. M., "Homology-dependent gene silencing in transgenic plants: epistatic silencing loci contain multiple copies of methylated transgenes" 244 : 219-229, 1994

72 Rodrigues, L. R., "Histology of embryogenic responses in soybean anther culture" 80 (80): 129-137, 2005

73 Cunha, W. G., "High resistance to Sclerotinia sclerotiorum in transgenic soybean plants transformed to express an oxalate decarboxylate gene" 59 : 654-660, 2010

74 Zhang, K., "GmRAV1 regulates regeneration of roots and adventitious buds by the cytokinin signaling pathway in Arabidopsis and soybean" 165 (165): 814-829, 2018

75 Zhao, G., "Germination of embryo in soybean anther culture" 43 (43): 1991-1995, 1998

76 Komatsuda, T., "Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max" 75 (75): 695-700, 1988

77 Phippen, C., "Genotype, plant, bud size and media factors affecting anther culture of cauliflowers (Brassica oleracea var. botrytis)" 79 : 33-38, 1990

78 Schmutz J, "Genome sequence of the palaeopolyploid soybean" 463 : 178-183, 2010

79 Eck, J. V, "Genome editing and plant transformation of solanaceous food crops" 49 : 35-41, 2018

80 Rhodes, C. A., "Genetically transformed maize plants from protoplast" 240 (240): 204-207, 1988

81 Herman, E. M., "Genetic modification removes an immunodominant allergen from soybean" 132 : 36-43, 2003

82 Kita, Y., "Genetic improvement of the somatic embryogenesis and regeneration in soybean and transformation of the improved breeding lines" 26 : 439-447, 2007

83 Qian, W., "Genetic effects on biomass yield in interspecific hybrids between Brassica napus and B. rapa" 134 : 9-15, 2003

84 Dufourmantel, N., "Generation and characterization of soybean and marker-free tobacco plastid transformants over-expressing a bacterial 4-hydroxyphenylpyruvate dioxygenase which provides strong herbicide tolerance" 5 : 118-133, 2007

85 Dufourmantel, N., "Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin" 58 : 659-668, 2005

86 Zheng, Q., "Gene regulation by the AGL15 transcription factor reveals hormone interactions in somatic embryogenesis" 172 (172): 2374-2387, 2016

87 Zhang, C., "Functional analysis of the GmESR1 gene associated with soybean regeneration" 12 (12): e0175656-, 2017

88 Chen, R., "Expression of Δ6, Δ5 desaturase and GLELO elongase genes from Mortierella alpina for production of arachidonic acid in soybean [Glycine max (L.) Merrill] seeds" 170 : 399-406, 2006

89 Cunha, N. B., "Expression of functional recombinant human growth hormone in transgenic soybean seeds" 20 : 811-826, 2011

90 Kim, W. S., "Expression of an 11kDa methionine-rich delta-zein in transgenic soybean results in the formation of two types of novel protein bodies in transitional cells situated between the vascular tissue and storage parenchyma cells" 2 : 199-210, 2004

91 Uchimiya, H., "Expression of a foreign gene in callus derived from DNA treated protoplasts of rice (Oryza sativa L.)" 22 : 421-477, 1986

92 Ferrie, A., "Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line" 14 : 580-584, 1995

93 Du, H., "Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9" 217 : 90-97, 2016

94 Zhang, W., "Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants" 76 : 835-840, 1998

95 Staswick, P. E., "Efficient down-regulation of the major vegetative storage protein genes in transgenic soybean does not compromise plant productivity" 127 (127): 1819-1826, 2001

96 Parrott, W. A., "Effect of genotype on somatic embryogenesis from immature cotyledons of soybean" 16 : 15-21, 1989

97 Lhee, W. Y., "Effect of BA and GA on embryo germination from ovule culture in intergeneric hybrids between Brassica and Raphanus" 24 : 257-262, 1997

98 Chiera, J. M., "Ectopic expression of a soybean phytase in developing seeds of Glycine max to improve phosphorus availability" 56 : 895-904, 2004

99 Farnham, M. W, "Doubled-haploid broccoli production using anther culture : effect of anther source and seed set characteristics of derived lines" 123 : 73-77, 1998

100 Finer, J. F., "Development of an embyrogenic suspension culture of soybean (Glycine max Merill)" 15 : 125-136, 1988

101 Shan, Z., "Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr)" 24 (24): 507-512, 2005

102 Neale D. B., "Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies" 15 : R59-, 2014

103 Kaltchuk-Santos, E., "Cytological analysis of early microspore divisions and embryo formation in cultured soybean anthers" 49 (49): 107-115, 1997

104 Bezouw, R., "Converging phenomics and genomics to study natural variation in plant photosynthetic efficiency" 97 (97): 112-133, 2019

105 Yang, Y. S., "Comparative studies of organogenesis and plant regeneration in various soybean explants" 72 (72): 101-108, 1990

106 Kaneda, Y., "Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybean (Glycine max (L.) Merrill)" 17 : 8-12, 1997

107 Eckert, H., "Co-expression of the borage Δ6 desaturase and the Arabidopsis Δ15 desaturase results in high accumulation of steridonic acid in the seeds of transgenic soybean" 224 : 1050-1057, 2006

108 Klein, A. S., "Cellulose and 1,3-glucan synthesis during the early stages of wall regeneration in soybean protoplasts" 152 (152): 105-114, 1981

109 Kao, K. N., "Cell division in newly formed cells from protoplasts of soybean" 62 : 338-340, 1970

110 Rakshit, S., "Callus induction and whole plant regeneration in elite indian maize (Zea mays L.) inbreds" 100 : 31-37, 2009

111 Bao, A., "CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9genes alters plant architecture in soybean" 19 : 131-, 2019

112 Cai, Y., "CRISPR/Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean" 16 : 176-185, 2018

113 Jaganathan, D., "CRISPR for crop improvement: An update review" 9 : 985-, 2018

114 Buter, B., "Autoclaved and filter sterilized liquid media in maize anther culture: significance of activated charocoal" 13 : 79-82, 1993

115 Lin, C. S., "Application of protoplast technology to CRISPR/Cas9mutagenesis: From single-cell mutation detection to mutant plant regeneration" 16 : 1295-1310, 2018

116 Guiderdoni, E., "Anther culture of tropical japonica x indica hybrids of rice (Oryza sativa L.)" 62 (62): 219-224, 1992

117 Hai, N. H., "Anther culture of Glycine max (Merr.): Effect of media on callus induction and organogenesis" 76 (76): 319-325, 2016

118 Ivers, D. R., "Anther culture in soybeans" 14 (14): 891-893, 1974

119 Dang, W., "An optimized Agrobacteriummediated transformation for soybean for expression of binary insect resistance genes" 173 : 381-389, 2007

120 Hamada, H., "An in planta biolistic method for stable wheat transformation" 7 : 11443-, 2017

121 Sahoo, K. K., "An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars" 7 : 49-, 2011

122 Zheng, Q., "Alterations in the transcriptome of soybean in response to enhanced somatic embryogenesis promoted by orthologs of Agamous-like15 and Agamouslike18" 164 (164): 1365-1377, 2014

123 Ishida, Y, "Agrobacteriummediated transformation of maize" 2 : 1614-1621, 2007

124 Yoshida, T, "Adventitious shoot formation from hypocotyl sections of mature soybean seeds" 52 : 1-8, 2002

125 Ochatt, S., "Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae)" 166 : 1314-1328, 2009

126 Ge, X. J., "A tissue culture system for different germplasms of indica rice" 25 : 392-402, 2006

127 Sairam, R. V., "A study on effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus" 75 : 79-85, 2003

128 Travella, S., "A comparison of transgenic barley lines produced by particle bombardment and Agrobacteriummediated techniques" 23 : 780-789, 2004

129 Zhao, Q., "5-azacytiding promotes shoot regeneration during Agrobacterium-mediated soybean transformation" 141 : 40-50, 2019