<P>Summary</P><P>Apicidin is a cyclic tetrapeptide produced by certain isolates of <I>Fusarium semitectum</I> and has been shown to inhibit Apicomplexan histone deacetylase. An apicidin-producing strain (KCTC16676) of the...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A107587221
2010
-
SCI,SCIE,SCOPUS
학술저널
456-466(11쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>Summary</P><P>Apicidin is a cyclic tetrapeptide produced by certain isolates of <I>Fusarium semitectum</I> and has been shown to inhibit Apicomplexan histone deacetylase. An apicidin-producing strain (KCTC16676) of the...
<P>Summary</P><P>Apicidin is a cyclic tetrapeptide produced by certain isolates of <I>Fusarium semitectum</I> and has been shown to inhibit Apicomplexan histone deacetylase. An apicidin-producing strain (KCTC16676) of the filamentous fungus was mutated using an <I>Agrobacterium tumefaciens</I>-mediated transformation, resulting in 24 apicidin-deficient mutants. Three of the mutants had a T-DNA insertion in a gene that encodes a non-ribosomal peptide synthetase (NRPS). Results of sequence, expression, and gene deletion analyses defined an apicidin biosynthetic gene cluster, and the NRPS gene was named as apicidin synthetase gene 1 (<I>APS1</I>). A 63 kb region surrounding <I>APS1</I> was sequenced and analysis revealed the presence of 19 genes. All of the genes including <I>APS1</I> were individually deleted to determine their roles in apicidin biosynthesis. Chemical analyses of the mutant strains showed that eight genes are required for apicidin production and were used to propose an apicidin biosynthetic pathway. The apicidin analogues apicidin E, apicidin D<SUB>2</SUB> and apicidin B were identified from chemical analysis of the mutants. The cluster gene <I>APS2</I>, a putative transcription factor, was shown to regulate expression of the genes in the cluster and overexpression of <I>APS2</I> increased apicidin production. This study establishes the apicidin biosynthetic pathway and provides new opportunities to improve the production of apicidin and produce new analogues.</P>