대장암 세포주에서 UCP 유전자 발현과 종양표지 인자로서의 기능 연구 = Characterization of Uncoupling Protein Gene Expression and Its Role as a MarkerMolecule in Human Colon Cancer Cells

목차 (Table of Contents)

• I. INTRODUCTION = 1
• II. Materials and Methods = 14
• 1. Cell culture = 14
• 2. Animals = 14
• 3. Tissue preparation of rats = 14
• 4. Plasma assay = 15
• 5. Transfection = 15
• 6. Analysis of mRNA expression = 15
• 7. Preparation of protein = 17
• 7-1. Mitochondrial fraction = 17
• 7-2. Immunoblotting assay = 18
• 8. Immunofluorescence = 19
• 9. Immunohistochemistry assay = 20
• 10. Cell cycle analysis = 20
• 11. Cell growth assay = 21
• 12. Analysis of mitochondrial transmembrane potential = 21
• 13. Caspase assay = 22
• 14. Cloning of human UCP1 = 23
• 15. Measurement of intracellular ROS production = 24
• III. RESULTS = 25
• 1. UCPs expression is increased by DHEA in brown adipose tissue (BAT) of rat = 25
• 2. Lipid metabolism related genes are increased by DHEA in rat BAT = 25
• 3. Level of UCPs expression by DHEA is balanced in other tissues = 31
• 4. UCP1 is expressed in colon cancer = 31
• 5. UCP1 is expressed in cultured colon cancer cell lines = 37
• 6. SW480 cells are overexpressed with UCP1 = 37
• 7. Rosiglitazone is increased PPARγexpression on SW480 cells = 40
• 8. Mitochondrial membrane potential is decreased on UCP1 overexpressed cells = 43
• 9. Overexpressed UCP1 induced apoptosis on cultured SW480 cells = 48
• 10. Overexpressed PGC1 induced apoptosis via UCP1 on SW480 cells = 51
• 11. UCP2 is expressed in SW480 colon cancer cell line = 54
• 12. Levels of Cox2 and IL6 mRNA expression was reduced by rosiglitazone in SW480 cells = 54
• 13. Overexpressed UCP2 reduced ROS production in SW480 cells = 57
• IV. DISCUSSION = 63
• REFERENCES = 70
• 국문 초록 = 82