산겨릅나무로부터 페놀화합물의 분리 및 항염증 활성의 측정 = Isolation and Identification of Phenol Compounds from Acer tegmentosum and their Anti-inflammatory Activity

다국어 초록 (Multilingual Abstract)

The Acer tegmentosum (3 kg) were extracted with boiled water and the freeze dried extract powder was partitionedwith CH2Cl2, EtOAc, n-BuOH and H2O, successively. From the EtOAc and n-BuOH fraction, six phenolic compounds wereisolated through the silica gel, octadecyl silica gel and sephadex LH-20 column chromatography. On the basis of spectroscopicmethods, such as1H-NMR and13C-NMR, and LC/MS, the chemical structures of the compounds as feniculin (1), avicularin(2), (+)-catechin (3), (-)-epicatechin (4), salidroside (5) and 6’-O-galloylsalidroside (6). In this study, compounds 1 and 2 havebeen first isolated from the A. tegmentosum. To provide insight into the effects of six compounds isolated from A. tegmentosumon inflammation, we investigated its effect on nitric oxide (NO) production in RAW 264.7 cells using lipopolysaccharide (LPS)stimulation. Compounds 1 and 6 slightly repressed NO production. Also, compounds 3 and 4 inhibited NO secretion with statisticalsignificance. However, compounds 2 and 5 did not show any inhibitory effect on NO production.

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