매미허물에서 분리한 키틴의 물리화학적 특성 = Physicochemical Characteristics of the Chitin Isolated from Cicadae Periostracum

다국어 초록 (Multilingual Abstract)

In this study, the physicochemical properties of the chitin isolated from cicadae (Platypleura kaempferi) periostracum was investigated. The prepared chitin was white flake form and the yield was 31.7%. The chitin was degraded into oligosaccharide during acid hydrolysis at 30oC with concentrated HCl. The hydrolysis rate was 6.8% only after 0.5 h reaction, but increased to 44.0% after 24 h reaction. In distribution of the oligosaccharides in the hydrolyzates, ratio of GlcNAc1-3 content increased from 66.5% after 0.5 h reaction to 84.4% after 12 h reaction. The ratio of monomer GlcNAc content was 79.4% after 24 h reaction and no oligosaccharides bigger than GlcNAc4 was detected. It is found that cicadae periostracum chitin belongs to α-chitin resulting from the FT-IR spectra similar with those of crab and shrimp chitins. In X-ray diffractometer, two peaks were exhibited at 2θ = 9.5o (interplanar distance : 9.36Å) and 2θ = 19.4o(4.58Å). The crystallinity index and crystal size of the chitin were determined to be 81.9% and 4.6 nm, respectively, which are similar with those of crab and shrimp chitins, again confirming that cicadae periostracum chitin belongs to α-chitin. The degree of acetylation of cicadae periostracum chitin was calculated to be 88.1%.

참고문헌 (Reference)

1 Scherrer, P, 2 : 96-100, 1918

2 김옥란, "토마토 초기 생장에 미치는 키틴 처리의 영향" 한국응용생명화학회 47 (47): 361-365, 2004

3 Nagahama, H, "XRD studies of $\beta$-chitin from squid pen with calcium solvent." 42 : 309-313, 2008

4 Rupley, J. A, "The hydrolysis of chitin by concentrated hydrochloric acid, and the preparation of low-molecular weight substrates for lysozyme" 83 : 245-255, 1964

5 Sannan, T, "Studies on chitin: 7. IR spectroscopic determination of degree of deacetylation." 19 : 458-459, 1978

6 Hackman, R. H, "Studies on chitin. I. Enzymic degradation of chitin and chitin esters" 7 : 168-178, 1954

7 Zhang, M, "Structure of insect chitin isolated from beetle larva cuticle and silkworm (Bombyx mori) pupa exuvia" 27 : 99-105, 2000

8 Lee, K. G, "Structural characteristics of insect chitin/chitosan" 40 : 158-162, 1998

9 Agull, E., "Present and future role of chitin and chitosan in food." 3 : 521-530, 2003

10 Horowitz, S. T, "Preparation of glucosamine oligosaccharides. 1. Separation" 79 : 5046-5049, 1957

11 A.O.A.C, "Official Methods of Analysis,"

12 Nemtsev, S. V, "Isolation of chitin and chitosan from honeybees" 40 : 39-43, 2004

13 Baxter, A, "Improved method for IR determination of degree of Nacetylation of chitosan" 14 : 166-169, 1992

14 Acosta, N, "Extraction and characterization of chitin from crustaceans" 5 : 145-153, 1993

15 Sagheera, F. A. A, "Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf" 77 : 410-419, 2009

16 Hahn, B. S, "Evaluation of anticoagulant and fibrinolytic activities from crude extract of insects" 30 : 409-412, 1999

17 Tamai, Y, "Enhanced healing of cartilaginous injuries by N-acetyl-D-glucosamine and glucuronic acid." 54 : 251-262, 2003

18 Gilbert, S. F, "Developmental Biol" Sinauer Associates Inc 2003

19 Broussignac, P, "Chitosan, a natural polymer not well known by the industry" 99 : 1241-1246, 1968

20 Roberts, G. R. T, "Chitin Chemistry" Macmilian Press 1992

21 Roberts, G. R. T, "Chitin Chemis" Macmilian Press 1992

22 Choi, H. Y, "Characteristic of chitosan prepared from α-, β-, and γ -chitin" 9 : 35-39, 2004

23 Im, S. H., "Antioxidative activity of some natural products which have been orientally used as ophthalmic drugs" 10 : 365-373, 2005

24 BeMiller, J. N, "Alkaline degradation of amino sugars" 27 : 1161-1164, 1962

25 Forcher, B, "Alkaline N-deacetylation of chitin enhanced by flash treatments reaction kinetics and structure modifications" 12 : 405-418, 1990

26 Tanaka, T, "A unique chitinase with dual active sites and triple substrate binding sites from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1" 65 : 5334-5338, 1999

27 Imoto, T, "A simple activity measurement of lysozyme" 35 : 1154-1156, 1971