포부자추출물의 갑상선기능저하증 흰쥐모델에서의 한열조절작용에 의한 개선효능 연구 = Effects study of Aconiti Lateralis Radix Preparata extract on the regulation of heat and cold in PTU-induced hypothyroidism rats

다국어 초록 (Multilingual Abstract)

Objective : To suggest a scientific evidence of Aconitum carmichaeli Debx. (Aconiti Lateralis Radix Preparata: ALRP) as one of cooling and heating medicines on the regulation of body temperature, we investigated the effects of ALRP water extract on hypothyroidism. Methods : Hypothyroidism was induced by intradermal injection with PTU for 4 weeks in SD rats. ALRP extract or L-thyroxine as a control drug was orally administrated for 2 weeks with PTU injection in rats. The physiological and serological parameters were measured in rats. The histological change of thyroid tissues was observed by H&E staining, and also the expression of thermo-regulating proteins was determined by Western blot in dorsal root ganglia and brain tissues of rats. Results : The administration of ALRP extract in PTU-induced hypothyroidism rats was significantly increased body temperature, but did not changes on body weight, food and water intake. ALRP extract did not effect on the levels of TSH and T4 in the hypothyroidism rats. ALRP extract significantly decreased the levels of GPT, glucose, HDL-cholesterol, LDL-cholesterol, and total cholesterol in the hypothyroidism rats. In histological observation, the enlarged epithelium and atrophic follicles with higher concentration of follicular cells on hypothyroidism were improved by ALRP extract. In addition, ALRP extract increased the expression of TRPV1 and TRPM8 ion channel proteins in hypothyroidism rats. Conclusion : These results indicate that ALRP extract can improve PTU-induced hypothyroidism through regulation of body temperature and lipid accumulation. The action mechanism of ALRP extract is related with body temperature control by thermoregulation with TRP ion channels.

참고문헌 (Reference)

1 김승모, "인삼이 PTU (6-n-propyl-2-thiouracil)로 유발된 Rat 갑상선 기능저하증에 미치는 영향" 대한본초학회 25 (25): 11-18, 2010

2 이승진, "육종용이 PTU로 유발된 갑상선 기능저하증 동물모델에 미치는 영향" 한의병리학회 25 (25): 989-995, 2011

3 김승모, "시호가 PTU (6-n-propyl-2-thiouracil)로 유발된 Rat 갑상샘 기능저하증에 미치는 영향" 한의병리학회 26 (26): 714-723, 2012

4 이상헌, "附子가 6-propyl-2-thiouracil(PTU)로 유발된 rat의 갑상선 기능저하증에 미치는 영향" 대한한방내과학회 28 (28): 275-283, 2007

5 구진숙, "生肝健脾湯加味方을 이용한 갑상선기능저하증 치험1例" 대한본초학회 29 (29): 17-21, 2014

6 홍민진, "淫羊藿이 실험적으로 유발된 갑상선 기능저하증 동물모델에 미치는 영향과 안전성" 대한약침학회 14 (14): 13-22, 2011

7 Han JH, "pharmacology of Oriental medicine" Euiseongdang 271-278, 2004

8 Reed Larsen P, "Williams textbook of endocrinology" Saunders 333-, 2003

9 Escobar-Morreale HF, "Treatment of hypothyroidism with levothyroxine or a combination of levothyroxine plus L-triiodothyronine" 29 (29): 57-75, 2015

10 Wiersinga WM, "Thyroid hormone replacement therapy" Horm Res 74-81, 2001

11 Ahn SY, "Thyroid Clinic" Seongbosa 210-213, 2004

12 Roti E, "The use and misuse of thyroid hormone" 14 (14): 401-424, 1993

13 Werner SC, "The thyroid: a fundamental and clinical text" Harper & Row 1978

14 Cho BY, "The clinical thyroidology" Korea Medicine 437-476, 2010

15 Kim SI, "The clinical efficacy of Anjunlizongtang for thyroid patients" 65-72, 2004

16 Park HJ, "The Introduction of sanghanron" Yakupshinmunsa 184-216, 1995

17 Doo HK, "Study on the Kidney System of the Eastern Medicine" Institute of Oriental Medicine 1059-1065, 1993

18 Ahn DG, "Study of Processing of Herbal Medicines" Iljongsa 205-210, 1997

19 Takaishi M, "Reciprocal effects of capsaicin and menthol on thermosensation through regulated activities of TRPV1 and TRPM8" 66 (66): 143-155, 2016

20 최재영, "PTU(6-n-propyl-2-thiouracil)로 유발된 Rat 갑상선 기능저하증에 미치는 烏梅의 효과" 대한본초학회 30 (30): 109-119, 2015

21 Sarandöl E, "Oxidative stress and serum paraoxonase activity in experimental hypothyroidism： effect of vitamin E supplementation" 23 : 1-8, 2005

22 Kim HC, "Oriental medicine pharmacology" Jipmundang 372-374, 2001

23 Doo HK, "Oriental Medicine of Nephrology" The Academy of oriental Medicine 729-, 1993

24 Hardie RC, "Novel Ca2+ channels underlying transduction in Drosophila photoreceptors: implications for phosphoinositide-mediated Ca2+ mobilization" 16 (16): 371-376, 1993

25 Roberts CG, "Hypothyroidism" 363 (363): 793-803, 2004

26 The National College of Oriental Medicine Herbology Classroom, "Herbology" Youngrimsa 372-374, 2016

27 Pogatzki-Zahn EM, "Heat hyperalgesia after incision requires TRPV1and is distinct from pure inflammatory pain" 115 (115): 296-307, 2005

28 Choudhury S, "Experimentally induced hypo- and hyper-thyroidism influence on the antioxidant defence system in adult rat testis" 35 : 131-140, 2003

29 Lee MY, "Experimental study on the standardization of the Hot and the Cold Natures" 2 (2): 506-513, 1996

30 O'Connor JC, "Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators" 69 : 79-91, 2002

31 Zulewski H, "Estimation of tissue hypothyroidism by a new clinical score : evaluation of patients with various grades of hypothyroidism and controls" 82 (82): 771-776, 1997

32 Oner J, "Effect of vitamin E on follicular cell proliferation and expression of apoptosis-associated factors in rats with 6-N-propyl-2-thiouracil-induced goitrogenesis" 41 : 213-217, 2003

33 Clyde PW, "Combined levothyroxine plus liothyronine compared with levothyroxine alone in primary hypothyroidism: a randomized controlled trial" 290 (290): 2952-2958, 2003

34 Jo BY, "Clinical Thytoidology" Korea Medical Book Publisher 231-255, 2001

35 Hwangbo WH, "Clinical Study of Acute Aconite Poisoning" 25 : 1223-1228, 1982

36 Ra CS, "A Study on Bu-ja<附子>, Oh-du <烏頭> Toxicosis" 16 (16): 451-457, 1995