RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      아카시아 培養 Callus에서 原形質體 分離에 미치는 培地窒素의 影響 = Effects of medium-nitrogen sources on isolation of protoplasts from acacia cultured callus

      한글로보기

      https://www.riss.kr/link?id=A19624352

      • 0

        상세조회
      • 0

        다운로드
      서지정보 열기
      • 내보내기
      • 내책장담기
      • 공유하기
      • 오류접수

      부가정보

      다국어 초록 (Multilingual Abstract)

      The growth and protoplast-isolation from epicotyl induced callus of Robinia pseudoacacia L were investigated from B_5-plot containing nitrate and ammonium, NO_3-plot, NH_4-plot and nitrogen free(NF)-plot.
      Callus growth was the most in B_5-plot, and the least in NF-plot. For the isolation of protoplasts, 3 % cellulase, 1 % macerozyme, 13 % mannitol and 1 % sorbitol were used as the enzyme solution. The yield of protoplasts was the highest in the ammonium plot showing the maximum value of 7.0 ×10^5/gm. fresh weight from the callus after 15 days of the culture. And the others less than NH_4-plot in the yield of protoplasts, revealed the maximum values in the order of NF-, NO_3 -and B_5 plot. The incubation time for the highest yield of protoplasts from each was 3,4 5 and 6 hours for NH_4-, NF-, NO_3 -and B_5_plot, respectively.
      This result implies that the nitrate in the growht media lowers the isolation rate when a cultured callus is used for the protoplast-isolation.
      번역하기

      The growth and protoplast-isolation from epicotyl induced callus of Robinia pseudoacacia L were investigated from B_5-plot containing nitrate and ammonium, NO_3-plot, NH_4-plot and nitrogen free(NF)-plot. Callus growth was the most in B_5-plot, and t...

      The growth and protoplast-isolation from epicotyl induced callus of Robinia pseudoacacia L were investigated from B_5-plot containing nitrate and ammonium, NO_3-plot, NH_4-plot and nitrogen free(NF)-plot.
      Callus growth was the most in B_5-plot, and the least in NF-plot. For the isolation of protoplasts, 3 % cellulase, 1 % macerozyme, 13 % mannitol and 1 % sorbitol were used as the enzyme solution. The yield of protoplasts was the highest in the ammonium plot showing the maximum value of 7.0 ×10^5/gm. fresh weight from the callus after 15 days of the culture. And the others less than NH_4-plot in the yield of protoplasts, revealed the maximum values in the order of NF-, NO_3 -and B_5 plot. The incubation time for the highest yield of protoplasts from each was 3,4 5 and 6 hours for NH_4-, NF-, NO_3 -and B_5_plot, respectively.
      This result implies that the nitrate in the growht media lowers the isolation rate when a cultured callus is used for the protoplast-isolation.

      더보기

      동일학술지(권/호) 다른 논문

      분석정보

      View

      상세정보조회

      0

      Usage

      원문다운로드

      0

      대출신청

      0

      복사신청

      0

      EDDS신청

      0

      동일 주제 내 활용도 TOP

      더보기

      주제

      연도별 연구동향

      연도별 활용동향

      연관논문

      연구자 네트워크맵

      공동연구자 (7)

      유사연구자 (20) 활용도상위20명

      이 자료와 함께 이용한 RISS 자료

      나만을 위한 추천자료

      해외이동버튼