The growth and protoplast-isolation from epicotyl induced callus of Robinia pseudoacacia L were investigated from B_5-plot containing nitrate and ammonium, NO_3-plot, NH_4-plot and nitrogen free(NF)-plot.
Callus growth was the most in B_5-plot, and t...
The growth and protoplast-isolation from epicotyl induced callus of Robinia pseudoacacia L were investigated from B_5-plot containing nitrate and ammonium, NO_3-plot, NH_4-plot and nitrogen free(NF)-plot.
Callus growth was the most in B_5-plot, and the least in NF-plot. For the isolation of protoplasts, 3 % cellulase, 1 % macerozyme, 13 % mannitol and 1 % sorbitol were used as the enzyme solution. The yield of protoplasts was the highest in the ammonium plot showing the maximum value of 7.0 ×10^5/gm. fresh weight from the callus after 15 days of the culture. And the others less than NH_4-plot in the yield of protoplasts, revealed the maximum values in the order of NF-, NO_3 -and B_5 plot. The incubation time for the highest yield of protoplasts from each was 3,4 5 and 6 hours for NH_4-, NF-, NO_3 -and B_5_plot, respectively.
This result implies that the nitrate in the growht media lowers the isolation rate when a cultured callus is used for the protoplast-isolation.