Characterization of Antibacterial Strains against Kiwifruit Bacterial Canker Pathogen

다국어 초록 (Multilingual Abstract)

Kiwifruit (Actinidia spp.) is an economically important crop and a bacterial canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa), is the most destructive disease in kiwifruit production. Therefore, prevent and control of the disease is a critical issue in kiwifruit industry worldwide. Unfortunately, there is no reliable control methods have been developed. Recently, interest in disease control using microbial agents is growing. However, kiwifruit microbiota and their roles in the disease control is mainly remaining unknown. In this study, we secured bacterial libraries from kiwifruit ecospheres (rhizosphere, endospere, and phyllosphere) and screened reliable biocontrol strains against Psa. As the results, Streptomyces racemochromogenes W1SF4, Streptomyces sp. W3SF9 and S. parvulus KPB2 were selected as anti-Psa agents from the libraries. The strains showed forcible antibacterial activity as well as exceptional colonization ability on rhizosphere or phyllosphere of kiwifruit. Genome analyses of the strains suggested that the strains may produce several anti-Psa secondary metabolites. Our results will contribute to develop biocontrol strains against the kiwifruit canker pathogen and the disease management strategies.

목차 (Table of Contents)

• Materials and Methods Results Discussion

참고문헌 (Reference)

1 김은경, "키위를 첨가한 막걸리의 품질특성" 한국식품영양과학회 42 (42): 1821-1828, 2013

2 Louden BC, "Use of blue agar CAS assay for siderophore detection" 12 : 51-53, 2011

3 Baldani JI, "The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media : a practical guide for microbiologists" 384 : 413-431, 2014

4 Blin K, "The antiSMASH database version 2 : a comprehensive resource on secondary metabolite biosynthetic gene clusters" 47 : D625-D630, 2018

5 Aziz RK, "The RAST Server : rapid annotations using subsystems technology" 9 : 75-, 2008

6 Bulgarelli D, "Structure and functions of the bacterial microbiota of plants" 64 : 807-838, 2013

7 Hye Sun Kim, "Screening and investigation Lactobacillus spp. to improve Secale cereale silage quality" Wiley 88 (88): 1538-1546, 2017

8 Xin X-F, "Pseudomonas syringae : what it takes to be a pathogen" 16 : 316-328, 2018

9 Hardoim PR, "Properties of bacterial endophytes and their proposed role in plant growth" 16 : 463-471, 2008

10 Vacheron J, "Plant growth-promoting rhizobacteria and root system functioning" 4 : 356-, 2013

11 Kaur H, "Plant growth promoting rhizobacteria : a boon to agriculture" 5 : 17-22, 2016

12 Bringel F, "Pivotal roles of phyllosphere microorganisms at the interface between plant functioning and atmospheric trace gas dynamics" 6 : 486-, 2015

13 Bonanomi G, "Organic amendments, beneficial microbes, and soil microbiota : toward a unified framework for disease suppression" 56 : 1-20, 2018

14 H. SAWADA, "Novel biovar (biovar 6) of Pseudomonas syringae pv. actinidiae causing ­bacterial canker of kiwifruit (Actinidia deliciosa) in Japan." The Phytopathological Society of Japan 82 (82): 101-115, 2016

15 Miyashiro S, "New streptothricin-group antibiotics, AN-201 I and II" 36 : 1638-1643, 1983

16 Donati I, "New insights on the bacterial canker of kiwifruit(Pseudomonas syringae pv" 4 : 53-67, 2014

17 Pikovskaya RI, "Mobilization of phosphorus in soil in connection with the vital activity of some microbial species" 17 : 362-370, 1948

18 English AC, "Mind the gap : upgrading genomes with Pacific Biosciences RS long-read sequencing technology" 7 : e47768-, 2012

19 Wang DS, "Microwave irradiation is a useful tool for improving isolation of Actinomycetes from soil" 82 : 102-110, 2013

20 Cha J-Y, "Microbial and biochemical basis of a Fusarium wilt-suppressive soil" 10 : 119-129, 2016

21 Nakazawa Y, "Large-scale production of phospholipase D from Streptomyces racemochromogenes and its application to soybean lecithin modification" 165 : 1494-1506, 2011

22 Patel D, "Isolation and screening of phosphate solubilizing bacteria from sunflower rhizosphere" 2 : 438-441, 2013

23 Deepthi MK, "Isolation and screening of Streptomyces sp. from Coringa mangrove soils for enzyme production and antimicrobial activity" 2 : 110-116, 2012

24 Paranthaman S, "Intergeneric conjugation in Streptomyces peucetius and Streptomyces sp strain C5 : chromosomal integration and expression of recombinant plasmids carrying the chiC gene" 69 : 84-91, 2003

25 X. Yang, "Identification of Pseudomonas syringae pv.actinidiae strains causing bacterial canker of kiwifruit in the Anhui Province of China, and determination of their streptomycin sensitivities" Genetics and Molecular Research 14 (14): 8201-8210, 2015

26 Ohnishi S, "Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO13350" 190 : 4050-4060, 2008

27 Fujikawa T, "Genome analysis of the kiwifruit canker pathogen Pseudomonas syringae pv. actinidiae biovar 5" 6 : 21399-, 2016

28 Ahn S-J, "Experimental antimicrobial orthodontic adhesives using nanofillers and silver nanoparticles" 25 : 206-213, 2009

29 Elena Colombi, "Evolution of copper resistance in the kiwifruit pathogen Pseudomonas syringae pv. actinidiae through acquisition of integrative conjugative elements and plasmids" Wiley 19 (19): 819-832, 2017

30 Gan Kisaki, "Evaluation of various cultivars of Actinidia species and breeding source Actinidia rufa for resistance to Pseudomonas syringae pv. actinidiae biovar 3" Springer Science and Business Media LLC 84 (84): 399-406, 2018

31 V. K. Chebotar, "Endophytic bacteria in microbial preparations that improve plant development (review)" Pleiades Publishing Ltd 51 (51): 271-277, 2015

32 Balestra GM, "Current status of bacterial canker spread on kiwifruit in Italy" 4 : 34-36, 2009

33 Wilson K., "Current protocol in molecular biology" Wiley 2.4.1-2.4.5, 2001

34 Pulsawat N, "Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae" 393 : 31-42, 2007

35 Olano C, "Biosynthesis of the angoiogenesis inhibitor borrelidin by Streptomyces parvulus Tü4055 : cluster analysis and assignment of function" 11 : 87-97, 2004

36 Sébastien M, "Biological control in the microbiome era : challenges and opportunities" 89 : 98-108, 2015

37 Stone BWG, "Biogeographic patterns between bacterial phyllosphere communities of the southern magnolia(Magnolia grandiflora)in a small forest" 71 : 954-961, 2016

38 Renzi M, "Bacterial canker on kiwifruit in Italy : anatomical changes in the wood and in the primary infection sites" 102 : 827-840, 2012

39 De Jong W, "Attachment of Streptomyces coelicolor is mediated by amyloidal fimbriae that are anchored to the cell surface via cellulose" 73 : 1128-1140, 2009

40 Chi F, "Ascending migration of endophytic rhizobia, from roots to leaves, inside rice plants and assessment of benefits to rice growth physiology" 71 : 7271-7278, 2005

41 Cwynar LC, "An inexpensive sieving method for concentrating pollen and spores from fine-grained sediments" 16 : 1115-1120, 1979