Population Genetic Structure of the Bumblebee, Bombus ignitus (Hymenoptera: Apidae), Based on Mitochondrial COI Gene and Nuclear Ribosomal ITS2 Sequences

다국어 초록 (Multilingual Abstract)

The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is widely utilized for greenhouse pollination in South Korea. Understanding the magnitude of genetic diversity and geographic relationships is of fundamental importance for long term preservation and utilization. As a first step, we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to the “DNA barcode” region and the complete internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA from 88 individuals collected in nine South Korean localities. The complete ITS2 sequences were longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated 112-bp long repeats. The 658-bp long mtDNA sequences provided only six haplotypes with a maximum sequence divergence of 0.61% (4 bp), whereas the ITS sequences provided 84 sequence types with a maximum sequence divergence of 1.02% (21 sites). The combination of the current COI data with those of published data suggest that the B. ignitus in South Korea and China are genetically a large group, but those in Japan can be roughly separated into another group. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population were found to exist among the South Korean populations of B. ignitus , which suggests panmixia. This finding is consistent with our understanding of the dispersal capability of the species.

참고문헌 (Reference)

1 Keller A, "rRNA interaction and HMM-based ITS2 annotation" 430 : 50-57, 2009

2 Fitch WM, "Toward defining the course of evolution: minimal change for a specific tree topology" 20 : 406-416, 1971

3 Alam MT, "The type and mysorensis forms of the Anopheles stephensi (Diptera: Culicidae) in India exhibit identical ribosomal DNA ITS2 and domain-3 sequences" 103 : 75-80, 2008

4 Marcilla A, "The ITS-2 of the nuclear rDNA as a molecular marker for populations, species and phylogenetic relationships in Triatominae (Hemiptera: Reduviidae), vectors of Chagas disease" 18 : 136-142, 2001

5 Yoon HJ, "Temperature and humidity favorable for colony development of the indoor-reared bumblebee, Bombus ignitus" 37 : 419-423, 2002

6 Mikkola K, "Spring migrations of wasps and bumble bees on southern coast of Finland(Hymenoptera Vespidae and Apidae)" 44 : 10-26, 1978

7 Gómez-Zurita J, "Sequence, secondary structure and phylogenetic analyses of the ribosomal internal transcribed spacer 2 (ITS2) in the Timarcha leaf beetles (Coleoptera: Chrysomelidae)" 9 : 591-604, 2000

8 De La Rúa P, "Sequence and RFLP analysis of the ITS2 ribosomal DNA in two Neotropical social bees, Melipona beecheii and Melipona yucatanica(Apidae, Meliponini)" 54 : 418-423, 2007

9 Castresana J, "Selection of conserved blocks from multiple alignments for their use in phylogenetic tool" 8 : 669-674, 2000

10 Hackett BJ, "Ribosomal DNA internal transcribed spacer(ITS2)sequences differentiate Anopheles funestus and Anopheles and A. rivulorum, and uncover a cryptic taxon" 9 : 369-374, 2000

11 Kawamura Y, "Recent progress in paleontological studies on the Quaternary mammals of Japan" 47 : 107-114, 2006

12 Hartl DL, "Principles of population genetics" Sinauer Sunderland 1989

13 Mitsuhata M, "Pollination of crops with bumblebee colonies in Japan" 21 : 17-25, 2000

14 Hung YT, "Phylogenetic utility of the ribosomal internal transcribed spacer 2 in Strumigenys spp. (Hymenoptera: Formicidae)" 32 : 407-415, 2004

15 Wagener B, "Phylogenetic study of Diadegma species (Hymenoptera: Ichneumonidae) inferred from analysis of mitochondrial and nuclear DNA sequences" 37 : 131-140, 2006

16 Marinucci M, "Phylogenetic relationships of seven palearctic members of the maculipennis complex inferred from ITS2 sequence analysis" 8 : 469-480, 1999

17 Weekers PHH, "Phylogenetic relationships inferred from ribosomal ITS sequences and biogeographic patterns in representatives of the genus Calopteryx (Insecta: Odonata) of the West Mediterranean and adjacent West European zone" 20 : 89-99, 2001

18 Muccio T, "Phylogenetic analysis of Phlebotomus species belonging to the subgenus Larrousius (Diptera: Psychodidae) by ITS2 rDNA sequences" 30 : 387-393, 2000

19 Swofford DL, "PAUP*. Phylogenetic Analysis Using Parsimony (*and other method) software ver. 4.10, Sinauer Associates"

20 Huelsenbeck JP, "MrBayes : Bayesian inference of phylogeny" 17 : 754-755, 2001

21 Lee ML, "Morphological and DNA variabilities of Bombus ignitus Smith (Apodae : Hymenoptera)" 21 : 139-144, 2006

22 Vobis M, "Molecular phylogeny of isolates of Ctenocephalides felis and related species based on analysis of ITS1, ITS2 and mitochondrial 16S rDNA sequences and random binding primers" 94 : 219-226, 2004

23 Erasmus JC, "Molecular phylogeny of fig wasp pollinators(Agaonidae, Hymenoptera)of Ficus section Galoglychia" 36 : 61-78, 2007

24 Thanwisai A, "Molecular phylogeny of black flies (Diptera: Simuliidae) from Thailand, using ITS2 rDNA" 128 : 177-204, 2006

25 Posada D, "Modeltest : testing the model of DNA substitution" 14 : 817-818, 1998

26 Mukabayire O, "Mitochondrial and ribosomal internal transcribed spacer(ITS2)diversity of the African malaria vector Anopheles funestus" 8 : 289-297, 1999

27 Min Jee Kim, "Mitochondrial DNA sequence variation of the bumblebee, Bombus ardens (Hymenoptera: Apidae)" 한국응용곤충학회 12 (12): 133-139, 2009

28 Hwa Young Kim, "Mitochondrial DNA Sequence Variation of the Mason Bee, Osmia cornifrons (Hymenoptera: Apidae)" 한국잠사학회 16 (16): 75-86, 2008

29 Bandelt HJ, "Median-joining networks for inferring intraspecific phylogenies" 6 : 37-48, 1999

30 Katoh K, "MAFFT : a novel method for rapid multiple sequence alignment based on fast Fourier transform" 30 : 3059-3066, 2002

31 Iwasaki M, "Introduction of commercial bumblebees into Japan" 16 : 17-21, 1995

32 Tang JM, "Intraspecic heterogeniety of rDNA internal transcribed spacer in the Simu-lium damnosum (Diptera: Simulidae) complex" 13 : 244-252, 1996

33 Hyung Joo Yoon, "Interspecific Hybridization of the Bumblebees Bombus ignitus and B. terrestris" 한국잠사학회 18 (18): 41-48, 2009

34 Hyung Keun Oh, "ITS2 Ribosomal DNA Sequence Variation of the Bumblebee, Bombus ardens (Hymenoptera: Apidae)" 한국유전학회 31 (31): 293-303, 2009

35 Stouthamer R, "ITS-2 sequences do not differ for the closely related species Trichogramma minutum and T. platneri" 95 : 105-111, 2000

36 Holsinger KE, "Hierarchical analysis of nucleotide diversity in geographically structured populations" 142 : 629-639, 1996

37 Tokoro S, "Geographic variation in mitochondrial DNA of Bombus ignitus(Hymenoptera, Apidae)" 45 : 77-87, 2010

38 Shao ZY, "Genetic structure of Asian population of Bombus ignitus" 95 : 46-52, 2004

39 Samara R, "Genetic divergence of Trichogramma aurosum Sugonjaev and Sorokina (Hymenoptera: Trichogrammatidae) individuals based on ITS2 and AFLP analysis" 132 : 230-238, 2008

40 Ji Y, "Evolutionary conservation and versatility of a new set of primers for amplifying the ribosomal internal transcribed spacer regions in insects and other invertebrates" 3 : 581-585, 2003

41 Inoue MN, "Displacement of Japanese native bumblebee by the recently introduced Bombus terrestris(L. )(Hymenoptera, Apidae)" 12 : 135-146, 2008

42 Beebe NW, "DNA sequence analysis of the ribosomal DNA ITS2 region for the Anopheles punctulatus group of mosquitoes" 8 : 381-390, 1999

43 Hajibabaei M, "DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics" 23 : 167-172, 2007

44 Felsenstein J, "Confidence limits on phylogenics: an approach using the bootstrap" 29 : 783-791, 1985

45 Kawecki TJ, "Conceptual issues in local adaptation" 7 : 1225-1241, 2004

46 Hebert PDN, "Biological identifications through DNA barcodes" 7 : 313-321, 2003

47 Excoffier L, "Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows" 10 : 564-567, 2010

48 Huson DH, "Application of phylogenetic networks in evolutionary studies" 23 : 254-267, 2006

49 Dohzono I, "Alien bumblebee affects native plant reproduction through interactions with native bumblebee" 89 : 3082-3092, 2008

50 Kimura M, "A sample method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences" 16 : 111-120, 1980

51 Lanave C, "A new method for calculating evolutionary substitution rates" 20 : 86-93, 1984

52 Akaike H, "A new look at the statistical model identification" 19 : 716-723, 1974