The effect of extract from leaves and stalks of Angelica gigas on the innate immunity = The effect of extract from leaves and stalks of Angelica gigas on the innate immunity

다국어 초록 (Multilingual Abstract)

The dried root of Angelica gigas (A. gigas) has been traditionally used as an oriental medicine, which is known to improve blood circulation and blood stasis. In the present study, leaves and stalks of A. gigas were used to investigate their effects on the innate immunity. The extracts were prepared from leaves and stalks of A. gigas and were fed to mice. The numbers of blood cells, total WBCs, neutrophils, lymphocytes, eosinophils and basophils were increased by 50% in mice fed with leaves extract of A. gigas compared to control mice. However, the numbers of blood cells were decreased when treated with stalks extract of A. gigas. The level of cholesterol and triglyceride in serum was markedly reduced in both mice group fed with leaves extract and stalks extract of A. gigas compared to control group (P＜0.01). There was no significant change in the level of albumin, total protein, phosphate and calcium in serum. Activity of cationic peptide was found to be diffused in the testicles of mice fed with leaves extract of A. gigas compared to control group, which might be due to increased lysozyme in testicle. The lysoplate assay and immunohistochemistry assay suggest that the extract of leaves and stalks of A. gigas are immunogenic, but the effects might be related with acquired immune response rather than innate immunity.

참고문헌 (Reference)

1 Shin S, "inflammatory effects of an ethanol extract of Angelica gigas in a Carrageenan-air pouch inflammation model" 58 : 431-436, 2009

2 Ahn MJ, "The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry" 46 : 258-266, 2008

3 Canfield RE, "The amino acid sequence of egg white Lysozyme" 238 : 2698-2707, 1963

4 Phillips DC, "The Three-dimensional structure of an enzyme molecule" 215 : 78-90, 1966

5 Chi HJ, "Studies on the components of umbelliferate plants in Korea: pharmacological study of decursin, decursinol and nodakenin" 1 : 25-32, 1970

6 Osserman EF, "Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia" 124 : 921-952, 1966

7 Lowry OH, "Protein measurement with folin phenol reagent" 193 : 265-275, 1951

8 Hoffmann JA, "Phylogenetic perspectives in innate immunity" 284 : 1313-1318, 1999

9 Fleming A, "On a remarkable bacteriolytic element found in tissue and secretions" 93 : 306-317, 1922

10 Hankiewicz J, "Lysozyme in human fluid" 57 : 205-209, 1974

11 Kumazawa Y, "Lymphocyte activation by a polysaccharide fraction seperated from hot water extracts of Angelica acutiloba Kitakawa" 8 : 417-424, 1985

12 Charles AJ Jr, "Innate immune recognition" 20 : 197-216, 2002

13 Cole AM, "Innate antimicrobial activity of nasal secretions" 67 : 3267-3275, 1999

14 Kumazawa Y, "Immunostimulating polysaccharide seperated from hot water extract of Angelica acutiloba Kitakawa (Yamato tohki)" 47 : 75-83, 1982

15 Taesook Yoon, "Effects of Root Extracts from Angelica gigas and Angelica acutiloba on Inflammatory Mediators in Mouse Macrophages" 한국응용생명화학회 50 (50): 264-269, 2007

16 Vidal ML, "Development of an ELISA for quantifying lysozyme in hen egg white" 53 : 2379-2385, 2005

17 Choi SS, "Antinociceptive profiles of crude extract from roots of Angelica gigas Nakai in various pain models" 26 : 1283-1288, 2003

18 Ganz T, "Antimicrobial polypeptides" 75 : 34-38, 2004

19 Prager EM, "Animal lysozymes c and g: an overview" 75 : 9-31, 1996

20 Konstan MW, "A comparative study of lysozyme and its secretion by tracheal epithelium" 3 : 175-181, 1982