<i>Bacillus ginsengisoli</i> sp. nov., isolated from soil of a ginseng field

다국어 초록 (Multilingual Abstract)

<P>A novel bacterial strain DCY53<SUP>T</SUP> was isolated from a soil sample from a ginseng field and was characterized using a polyphasic approach. Cells were Gram-reaction-positive, rod-shaped, endospore-forming and motile with flagella. The strain was aerobic, catalase- and oxidase-positive, optimum growth temperature and pH were 30–37 °C and 6.0–7.5, respectively. On the basis of 16S rRNA gene sequence analysis, strain DCY53<SUP>T</SUP> was shown to belong to the genus <I>Bacillus</I> and the closest phylogenetic relatives were <I>Bacillus pocheonensis</I> KCTC 13943<SUP>T</SUP> (98.3 %), <I>Bacillus bataviensis</I> LMG 21833<SUP>T</SUP> (98.0 %), <I>Bacillus soli</I> LMG 21838<SUP>T</SUP> (97.9 %), <I>Bacillus drentensis</I> LMG 21831<SUP>T</SUP> (97.8 %), <I>Bacillus niacini</I> DSM 2923<SUP>T</SUP> (97.8 %), <I>Bacillus novalis</I> LMG 21837<SUP>T</SUP> (97.7 %), <I>Bacillus vireti</I> LMG 21834<SUP>T</SUP> (97.6 %) and <I>Bacillus fumarioli</I> LMG 17489<SUP>T</SUP> (97.3 %). The DNA G+C content was 43.6 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were iso-C<SUB>14 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>15 : 0</SUB>. The DNA–DNA relatedness with closest relatives was below 55 %. The results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that DCY53<SUP>T</SUP> represented a novel species within the genus <I>Bacillus</I>, for which we propose the name <I>Bacillus ginsengisoli</I>. The type strain is DCY53<SUP>T</SUP> ( = KCTC 13945<SUP>T</SUP> = JCM 17335<SUP>T</SUP>).</P>