삼백초의 Fyn/Gab2/PI3K 신호전달 경로 활성화 억제에 의한 비만세포 억제 효과

다국어 초록 (Multilingual Abstract)

Mast cells has a pivotal role in the induction of type I hypersensitive
response. In this study, the inhibitory effect and its mechanims of Saururi
herba (SH) on mast cells were investigated. The ethanol extract of SH
showed significant inhibitory effect on degranulation in antigen-stimulated
mast cells; its IC50 values were ~43 μg/ml for RBL-2H3 cells and -7 μ
g/ml for Bone marrow-derived mast cells (BMMC), and it also inhibited
the expression of TNF-α and IL-4 in antigen-stimulated RBL-2H3 cells.
As its mechanism of action, SH inhibited the phosphorylation of Gab2 and
PI3K/ Akt for the activation of mast cells. The results of the study suggest
that the inhibition of mast cells by SH is mediated by the inhibition of
Fyn/Gab2/PI3K pathway in mast cells.
Keywords : Saururi herba, mast cells, Gab2, PI3 kinase

목차 (Table of Contents)

• Ⅰ. 서론 2
• Ⅱ. 실험재료 및 방법 3
• 실험재료 3
• 삼백초의 에탄올 추출물 제조 3
• 생쥐 골수유래(BMMC) 및 RBL-2H3 비만세포의 분리 및 배양 3
• 비만세포에서 탈과립 측정 4
• Immunoblotting 분석 4
• Reverse transcription-polymerase chain reaction (RT-PCR) 5
• 실험결과의 통계처리 5
• Ⅲ. 실험결과 및 고찰 6
• 삼백초 추출물의 비만세포에서 탈과립에 대한 효과 6
• 삼백초 추출물의 TNF-α 및 IL-4 발현 억제 효과 6
• 삼백초추출물의 비만세포 억제 기전 6
• 삼백초추출물의 MAP kinase 활성화 억제 7
• Ⅳ. 결론 7