고유종 꼬리말발도리의 생식특성과 동위효소 유전다양성 = Division 1 ; Breeding System and Allozyme Genetic Diversity of Deutzia paniculata Nakai, an Endemic Shrub in Korea

다국어 초록 (Multilingual Abstract)

Deutzia paniculata is an endemic species, which is geographically restricted within southern part of Korea. Four populations of D. paniculata were sampled across its natural range, from the smallest population, Mt. Dalum,which held less than 100 individuals, to the largest, Mt. Unmum, over 3,500 individuals. Artificial pollination studyshowed that D. paniculata had an obligate outcross breeding system. Major pollinators were two bee species,Lasioglossum exiliceps and Allograpta balteata (de Geer). The breeding system and patterns of allozyme variation ofD. paniculata were investigated to understand the population biology and to explain on reserve designs and managementproposals relevant to this species. D. paniculata held relatively low genetic variation at the eight allozyme loci surveyed. Measures of genetic variation in this species alleles per locus (As=1.33), proportion of polymorphic loci (P=23.85%),and expected heterozygosity (Hes=0.110) were similar to values reported for endemic species. Mt. Dalum population(DAL) was composed with one clone based on allozyme data. Individuals of D. paniculata were frequently includedin root connected clusters. Population genetic structure between and within four populations was probably the result ofshrinking effective population size and the extinctions of intervening populations. For the conservation of geneticdiversity, maximum number of different genotype need to be protected based on genetic structure and mating system.

참고문헌 (Reference)

1 배기화, "제주 용암해수를 이용한 희귀식물 자란(Bletilla striata)의 기내배양" 한국식물생명공학회 39 (39): 281-287, 2012

2 박수경, "산림청 수목원 조성 및 진흥에 관한 법률의 특산식물 목록의 재고" 한국임학회 102 (102): 38-58, 2013

3 Chang, S.-M., "The role of inbreeding depression in maintaining the mixed mating system of the common morning glory, Ipomoea purpurea" 53 : 1366-1376, 1999

4 Coates, D.J., "The mating system and patterns of genetic variation Bankia cuneata A.S. George (Proteaceae)" 69 : 11-20, 1992

5 Wright, S., "The interpretation of population structure by F-statistics with special regard to systems of mating" 19 : 395-420, 1965

6 Wright, S., "The genetical structure of populations" 15 : 323-354, 1951

7 Fahselt, D., "The dangers of transplantation as a conservation technique" 8 : 238-241, 1988

8 Kephart, S.R., "Starch gel electrophoresis of plant isozyme: a comparative analysis of techniques" 75 : 1114-1119, 1990

9 Conkle, M.T., "Starch Gel Electrophoresis of Conifer Seeds: A laboratory Manual" USDA 1982

10 Chang, C. -S., "Reconsideration of rare and endangered plant species in Korea based on the IUCN Red List Categories" 31 : 107-142, 2001

11 Meffe, G.K., "Principles of Conservation Biology" Sinauer 1994

12 Given, D.R., "Principles and Practice of Plant Conservation" Timber Press 1994

13 Frankham, R., "Predicting the Probability of Outbreeding Depression" 25 (25): 465-475, 2010

14 Ellstrand, N.C., "Population genetic consequences of small population size: Implications for plant conservation" 24 : 217-242, 1993

15 Rathcke, B., "Pollination Biology" Academic Press 305-329, 1983

16 Hamrick, J.L., "Plant Population genetics, breeding and genetic resources" Sinauer 43-63, 1989

17 Pauli, H., "Plant Diversity Changes on Europe’s Mountain Summits" 336 (336): 353-355, 2012

18 Rathcke, B., "Phenological patterns of terrestrial plants" 16 : 179-214, 1985

19 Forest Service, "Pacific Southwest and Range Experiment Station"

20 Zaikonnikova. T. I., "Ornamental Shrubs. A Monographs of Genus Deuztia" Nauka 1966

21 Siegel, S., "Nonparamatic Statisctics" McGraw-Hill Kogakusha 1959

22 Chung, Y.H., "Monographic study of the endemic plants in Korea. VI. taxonomy and interspecific relationships of the Genus Deutzia" 29 : 207-231, 1986

23 Fiedler, P.L., "Life history and population dynamics of rare and common mariposa lilies (Calochortus: Liliaceae)" 75 : 977-995, 1987

24 Slatkin, M., "Isolation by distance in equilibrium and non-equilibruim populations" 47 : 264-279, 1993

25 Silvertown, J.W., "Introduction to plant population biology" Blackwell Science 1993

26 Delgado, D., "High population differentiation and genetic variation in the endangered Mexican pine Pinus rzedowskii (Pinaceae)" 86 : 669-676, 1999

27 Crow, J.F., "Group selection for a polygenic behavioral trait: Estimating the degree of population subdivision" 81 : 6073-6077, 1984

28 Barrett, S.C.H., "Genetics and Conservation of Rare Plants" Oxford University Press 3-30, 1991

29 Hamrick, J. L., "Genetics and Conservation of Rare Plants" Oxford University Press 76-86, 1991

30 Park, J., "Genetic variation in endangered Scrophularia takesimensis (Scrophulariaceae) from Ulleung Island" 51 : 371-376, 2010

31 Li, H., "Genetic diversity of the endangered Chinese endemic plant Monimopetalum chinense revealed by amplified fragment length polymorphism (AFLP)" 39 : 384-391, 2011

32 Aavik, T., "Genetic consequences of using seed mixtures in restoration: A case study of a wetland plant Lychnis flos-cuculi" 145 : 195-204, 2012

33 Chung, M. Y., "Extremely low levels of genetic variation in the critically endangered monotypic fern genus Mankyua chejuense (Ophioglossaceae) from Korea: Implications for conservation" 38 : 888-896, 2010

34 Nei, M., "Estimation of average heterogeniety and genetic distance a small number of individuals" 89 : 583-590, 1978

35 Young, B.E., "Endemic species distribution on the east slope of the Andes in Peru and Bolivia" NatureServe 2007

36 Hamrick, J.L., "Effects of life history traits on genetic diversity in plants species" 351 : 1291-1298, 1996

37 Baker, H.G., "Ecology of Soil Seed Banks" Academic Press 9-21, 1989

38 Avise, J.C., "Conservation genetics, case histories from nature" Campman and Hall 1996

39 Bouzat, J.L., "Conservation genetics of population bottlenecks:the role of chance, selection, and history" 11 (11): 463-478, 2010

40 Allendort, F. W., "Conservation and the Genetics of Populations" Wiley-Blackwell 2013

41 Rabinowitz, D., "Conservation Biology:The Science of Scarcity and Diversity" Sinauer Associates 182-204, 1986

42 Sipes, S.D., "Clonal structure and patterns of allozyme diversity in the rare endemic Cycladenia humilis var. jonesii (Apocynaceae)" 84 : 401-409, 1997

43 Wagenius, S., "Biparental inbreeding and interremnant mating in a perennial prairie plant: fitness consequences for progeny in their first eight years" 64 : 761-771, 2010

44 Kruckeberg, A.R., "Biological aspects of endemism in hight plants" 16 : 447-479, 1985

45 Sworford, D.L., "BIOSYS-1: a computer program for the analysis of allelic variation in population genetics and biochemical systematics" Illinois National History Survey 1989

46 Nei, M., "Analysis of gene diversity in subdivided populations" 70 : 3321-3323, 1973

47 Crow, J.F., "An introduction to population genetics theory" Harper and Row 1970

48 Liu, Y., "Allozyme variation of the endangered endemic plant Myricaria laxiflora:Implications for conservation" 38 : 463-470, 2010

49 Charleswarth, B., "A model for the evolution of dioecy and gynodioecy" 84 : 665-671, 1978