Capsicum annuum L. 배양세포의 Capsaicinoids 생성에 미치는 2. 4-Dichlorphenoxyactic acid 의 영향 = Effexts of 1, 4-Dichlorphenoxyactic acid on the Formation of Capsaicinoids in Callus of Capsicum annuu, L

다국어 초록 (Multilingual Abstract)

In order to investigate the effects of growth regulators on the formation of capsaicinoids in callus of capsicum annuum L., tissues were cultured in the Linsmaier and Skoog RM 1964 medium containing various growth regulators. Production of capsaicinoids during culture was monitored by gas chromatography-mass spectrometry and mass fragmentography, and the growth rate was measured by weighing the whole tissue.
Among growth regulators emploved, 2,4-D (10-6M) and kinetin (10-6M) were the most effective. The growth rate increased sharply from two to three weeks of culture periods, but slowed down afterwards; the final tissue weight after 25 days of culture was about six times the initial one. Effects of NAA and IAA were much inferior to that of 2,4-D. Furthermore almost no growth was observed in the presence of kinetin alone.
In the presence of 10-6M of 2, 4-D and kinetin in the medium, 1182㎍ of capsaicinoids were formed per 100g dry wt. of tissue, of which was greater than with any of three other growth regulators. IAA, NAA, and kinetin of same concentrations had 65%, 38%, 68% effect of 2,4-D in capsaicinoids formation respectively.
Production of capsainoids increased gradually in the presence of 2,4-D as culture period was proceeded. After 25 days of culture, amounts of capsaicinoids homologues produced were 498㎍ of capsaicin, 501㎍ of dihydrocapsaicin. and 183㎍ of nordihydrocapsaicin per 100g dry wt. of tissue.

목차 (Table of Contents)

• Ⅰ. 緖 論
• Ⅱ. 實驗材料 및 方法
• 1. Callus 誘起用 組織의 調製
• 2. 培養基 및 培養條件
• 3. 細胞의 生長率 測定
• 4. Capsaicinoids의 分離 및 定量
• Ⅲ. 結果 및 考察
• 1. Callus成長에 미치는 growth regulators의 影響
• 2. Capsaicinoids生長에 미치는 growth regulators의 影響
• Ⅳ. 要 約