Voltammetric Detection of Trimethylamine Using Immobilized Trimethylamine Dehydrogenase on an Electrodeposited Goldnanoparticle Electrode

다국어 초록 (Multilingual Abstract)

A voltammetric enzyme electrode was developed based on nicotinamide-independent trimethylamine dehydrogenase (TMADH, EC 1.5.99.7), which catalyses the oxidation of trimethylamine (TMA) to dimethylamine and formaldehyde. A quaternized osmium hydrogel polymer,poly(vinylimidazole-[Os(4,4'-dimethyl-2,2'-bipyridine)_2Cl]^(+/2+))with ethylamine (PVI-Os-EA), was prepared as a potential redox mediator in an electrochemical biosensor. TMA was detected using TMADH that was co-immobilized with an osmium hydrogel polymer on electrodeposited gold nanoparticles (Au-NPs) on screen-printed carbon electrodes (SPCEs). The Au-NPs deposited onto SPCEs provided about a three times higher electrochemical response compared to that of a planar gold electrode. As TMA was catalyzed by wired TMADH, the electrical signal was monitored at 0.3 V versus Ag/AgCl by cyclic voltammetry and chronoamperometry. The anode currents increased linearly in proportion to the TMA concentration over the 0~ 2.5 mM range with a detection limit of 1 μM (R =0.9972).

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