수종 한약제의 위암세포에 대한 항암작용 효능검색 및 약리작용에 관한 분자생물학적 연구 = Molecular analysis on the pharmacological actions of oriental medicines in stomach cancer cells

다국어 초록 (Multilingual Abstract)

Traditional oriental medicines have been used for treatment of various kinds of human cancers for long times and some of them proven to be effective clinically. However. the pharmacological actions and mechanisms related to cancer treatment are generally unknown. In an effort to clarify the action mechanisms of several oriental medicines used for cancer treatments, we planned this experimental procedures.
We selected Cordyceps sinensis (冬蟲夏草). Punellae Herba (夏枯草), Rehmanniae Radix (熟地黃), Paeoniae Radix (白芍約), Oldenlandiae Herba (白花蛇舌草), Portulacae Herba (馬齒?), Scolopendra subspinipes mutilans (蜈蚣), Mylabris Phalerata (斑?), Phellinus igniarius(桑黃), Ganodermae Lignum(靈芝) for evaluation, which have been used for patients of gastric cancers. The twenty grams of medicines were boiled in 100㎖ of water for 1 hour and filtered with 0.2 ㎛ pore-sized filter unit to remove insoluble particles.
Initially we evaluated the effects oriental medicines on growth inhibition in stomach cancer cells. The gastric cancer cell line, AGS, was cultured in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum and treated with 10% oriental medicines per 1 ㎖ of medium up to 48 hours. The specimens were subjected to MTT assay for evaluation of growth inhibition. We found that Mylabris phalerata (斑? ) markedly suppressed the growth of cancer cells as shown in results.
Next, we checked the effects of oriental medicines on cancer on cell cycles and apoptosis. Mylabris phalerata (斑?　) inhibited cell cycle progression of cancer cells as compared with control cells and cells treated with other medicines. In addition, Mylabris phalerata (斑?　) induced apoptosis in 30-40% of stomach cancer cells within 24 hours. Other oriental medicines used for this experiments did not show apoptosis-inducing effects on cancer cells.
Finally, we determined the expression levels of genes associated with cell cycle and apoptosis. The expressions of Bcl-2 and bcl-XL were downregulated by the treatments of Mylabris phalerata (斑?　), However, the expression levels of genes related to cell cycles were not altered significantly.
In conclusion, we found that Mylabris phalerata(斑?　) has in vitro growth-inhibiting and apoptosisinducing effectd on stomach cancer cells. However, we think that at least animal experiments are necessary for evaluations.