For the induction of somatic embryogenic callus, the various explants of Corydalis remota for. remota were cultured on MS basal media supplemented with 2,4-D, kinetin and zeatin. Embryogenic calli were obtained from the leaf, stem, penduncle and root ...
For the induction of somatic embryogenic callus, the various explants of Corydalis remota for. remota were cultured on MS basal media supplemented with 2,4-D, kinetin and zeatin. Embryogenic calli were obtained from the leaf, stem, penduncle and root explants after 20-60 days. The highest embryogenic callus formation was observed from leaf on the media containing 1.0 ㎎/L of 2,4-D and 1.0 ㎎/L of kinetin.
the somatic embryogenesis on the media with 0.1 ㎎/L of 2,4-D 2 ㎎/L of kinetin were excellent under light condition. Generally the somatic embryos were abnormally developed into plantlets. However, normal decotyledonary plantlets were found on MS medium supplemented with 1.0 ㎎/L of zeatin or kinetin. When MS medium with 2,4-D plus cytokinin and with BAP were used, the secondary somatic embryogenesis took place in root explants of the regenerants derived from in vitro somatic embryogenic callus.