미더덕의 생리활성이 향상된 아임계수 추출물의 제조

국문 초록 (Abstract)

미더덕의 이용 다변화를 위하여 아임계수 추출물을 제조하고 생리활성을 평가하였다. 미더덕은 동결건조 분말 상태로 10, 30, 60분 동안 다양한 온도(50, 100, 200, 300℃)에서 추출되었다. 아임계수 조건은 ACE 저해 활성, 항산화 활성, AChE 저해 활성과 같은 중요한 생리학적 특성을 상당히 증가시켰다. 200℃, 60분 추출물에서 가장 높은 ACE 저해활성을 나타냈으나 더 높은 온도 조건인 300℃에서는 급격히 감소하였다. DPPH 라디칼 소거능과 ABTS라디칼 소거능은 300℃, 30분 추출물에서 공통적으로 가장 높은 값을 보였고, AChE 저해 활성 또한 같은 경향을 나타냈다. 이러한 결과는 아임계수 추출이 미더덕의 생리활성을 증가시키는 유용한 가공방법이 될 수 있음을 시사한다.

다국어 초록 (Multilingual Abstract)

For diverse application of Styela clava (Korean name: miduduck) as a food material, subcritical water (SCW) extract was prepared and its physiological activity was evaluated. To accomplish this, S. clava powder (0.1 g) was placed in a stainless vessel containing 10 mL of distilled water, after which SCW extraction was carried out at 50, 100, 200, and 300℃ for 10, 30, and 60 min. SCW treatment significantly increased important physiological properties of the extract such as angiotensin converting enzyme (ACE) inhibitory activity, antioxidant activity, and acetylcholinesterase (AChE) inhibitory activity. The highest ACE inhibitory activity was found in samples subjected to SCW extraction at 200℃ for 60 min; however, the activity decreased at higher temperature (300℃). The SCW extract of S. clava prepared at 300℃ for 30 min showed the highest antioxidant activity [1,1-diphenyl-2-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities] and AChE inhibitory activity. These results indicate that SCW extraction might be a useful method for increasing the physiological activity of S. clava.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

1 이동원, "채취시기에 따른 미더덕의 부위별 항산화 활성 및 ACE 저해 활성" 한국식품영양과학회 39 (39): 331-336, 2010

2 이승철, "주름 미더덕(Styela plicata) 추출물의 항산화력 및 항암활성" 한국식품영양과학회 34 (34): 937-941, 2005

3 이승철, "암세포주에 대한 미더덕 추출물의 세포독성 효과" 한국식품영양과학회 35 (35): 823-827, 2006

4 정은실, "미더덕의 부위별 추출물의 항산화 활성" 한국식품영양과학회 37 (37): 1674-1678, 2008

5 배명숙, "미더덕을 첨가한 김치의 발효특성" 한국식품조리과학회 24 (24): 573-579, 2008

6 이승철, "미더덕과 오만둥이를 이용한 술의 제조 및 특성" 한국식품영양과학회 36 (36): 1038-1042, 2007

7 이승철, "미더덕 함유 어묵의 품질 특성" 한국식품영양과학회 35 (35): 908-911, 2006

8 신유진, "미더덕 껍질, 뽕잎, 양파 추출물을 첨가한 항고혈압 저염간장의 제조 및 특성" 한국식품영양과학회 43 (43): 854-858, 2014

9 정석문, "감귤 과피 추출물의 항산화능에 대한 원적외선 처리의 효과" 한국식품영양과학회 33 (33): 1580-1583, 2004

10 이승철, "가공방법 및 용매에 따른 미더덕 추출물의 항산화 및 항암효과" 한국식품영양과학회 35 (35): 278-283, 2006

11 Joh YG, "The sterol composition of Styela clava" 11 : 97-101, 1978

12 Jo EK, "The effects of subcritical water treatment on antioxidant activity of golden oyster mushroom" 6 : 2555-2561, 2013

13 Kim JW, "Subcritical water extraction of nutraceutical compounds from citrus pomaces" 44 : 2598-2608, 2009

14 Cushman DW, "Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung" 20 : 1637-1648, 1971

15 Pellegrini N, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2, 2’-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)radical cation decolorization assay" 299 : 379-389, 1999

16 Kus NS, "Organic reactions in subcritical and supercritical water" 68 : 949-958, 2012

17 Ko SC, "Nitric oxide-mediated vasorelaxation effects of anti-angiotensin Ⅰ-converting enzyme(ACE)peptide from Styela clava flesh tissue and its anti-hypertensive effect in spontaneously hypertensive rats" 134 : 1141-1145, 2012

18 Lee HJ, "Inhibitory activities of Basidiomycetes on prolyl endopeptidase, acetylcholine esterase and coagulation" 42 : 336-343, 1999

19 Baek JY, "Extraction of nutraceutical compounds from licorice roots with subcritical water" 63 : 661-664, 2008

20 Iroyukifujita H, "Classification and antihypertensive activity of angiotensin Ⅰ-converting enzyme inhibitory peptides derived from food proteins" 65 : 564-569, 2000

21 한지경, "Antioxidant, ACE inhibitory, and acetylcholinesterase inhibitory activities of subcritical water extract of blue mussel" 한국식품과학회 27 (27): 847-851, 2018

22 Seo HK, "Antioxidant activity of subcritical water extracts from Chaga mushroom(Inonotus obliquus)" 45 : 198-203, 2013

23 Kim SJ, "Antioxidant activity of a hydrothermal extract from watermelons" 59 : 361-368, 2014

24 신유진, "Antioxidant Activity and β-Glucan Contents of Hydrothermal Extracts from Maitake (Grifola frondosa)" 한국식품과학회 23 (23): 277-282, 2014

25 Lee SY, "Antihypertensive peptides from animal products, marine organisms, and plants" 228 : 506-517, 2017

26 Talesa VN, "Acetylcholinesterase in Alzheimer’s disease" 122 : 1961-1969, 2001

27 Carr AG, "A review of subcritical water as a solvent and its utilisation for the processing of hydrophobic organic compounds" 172 : 1-17, 2011