근적외선 분광 분석법을 이용한 참깨의 지방함량 측정 = Determination of Fatty Acid Composition of Sesame(Sesamum indicum) Seeds by Near-Infrared Reflectance Spectroscopy

다국어 초록 (Multilingual Abstract)

A methodological study was conducted to test the potential of near-infrared reflectance spectroscopy (NIRS) for the estimation of fatty acid composition and content in sesame seeds. A set of 3237 sesame lines, provided from the Rural Development Administration, were scanned by NIRS for their fatty acids. Calibration equations for the content of fatty acids were developed for the 122 lines of them by GC-FID(gas chromatography-flame ionization detector). Main fatty acids were palmitic acid(C(16:0), 8.5%), stearic acid(C(18:0), 5.7%), oleic acid(C(18:1), 39.3%), linoleic acid(C(18:2), 43.4%), and linolenic acid(C(18:3), 0.4%); the lowest and highest level were IT028899-7.2% and IT169359-10.0% in palmitic acid and IT028897-4.5%, IT028910-7.0% in stearic acid, IT170020-32.2% and IT156371-46.5% in oleic acid, IT156348-36.5 and IT169926-49.6% in linoleic acid, and IT169316-0.2% and IT156367-0.6% in linolenic acid, respectively. Determination of the fatty acid composition of seeds could be carried out successfully by using the spectra patterns of NIRS for both oleic and linoleic acids. As a result, oleic acid and linoleic acid compositions(% of total fatty acid) ranged from 25.8% to 49.2% and from 21.7% to 52.2%, respectively, in sesame seeds. Correlation(r²) between the percentage of oleic acid and linoleic acid was calculated in (-0.23).

참고문헌 (Reference)

1 EI Tinay, A.H, "Protein and oil compositions of sesame seed" 53 : 648-665, 1976

2 Yermanos, D.M, "Oil content and composition of the seed in the world collection of sesame introductions" 49 : 20-23, 1972

3 Tetsuo Sato, "Nondestructive determination of fatty acid composition of husked sunflower(Helianthus annua L.) seeds by Near Infrared spectroscopy" 72 (72): 1177-1183, 1998

4 Hoe-II chung, "NIR-Infrared Spectroscopy: Principles" 13 : 2000

5 Tetsuo Sato, "NIR Reflectance spectroscopic analysis of the FA composition in sesame( Sesamum indicum L.) seeds" 80 (80): 1157-1161, 2003

6 Seong In Cho, "Measurement of moisture and fat contents in pepper-powder using Near-Infrared spectroscopy" 1 (1): 133-136, 1997

7 김정봉, "Gas Chromatograph-Mass Spectrometer를 이용한 γ-Linolenic Acid(GLA) 생성 균주탐색 및 확인" 한국균학회 35 (35): 96-100, 2007

8 Brar, G.S, "Effect of temperature on fatty acid composition of sesame(Sesamum indicum L.)" 7 : 133-136, 1980

9 Han Bum Lee, "Determination of seed protein and oil concentration in kiddny bean by Near Infrared spectroscopic analysis" 46 (46): 248-252, 2001

10 B. Perez-vich, "Determination of seed oil content and fatty acid compositon in sunflower through the analysis of intact seeds, husked seeds, meal and oil by Near Infrared reflectance spectrocopy" 75 (75): 547-555, 1998

11 Su-Noh Ryu, "Current status and prospects of quality evaluation in sesame" 47 : 140-149, 2002