Reduction-responsive release property of egg phosphatidylcholine liposomes incorporating benzyl disulfide

다국어 초록 (Multilingual Abstract)

Reduction-responsive liposome was prepared by incorporating benzyl disulfide in egg phosphatidylcholine(egg PC) liposomal bilayer. Thefluorescence quenching degree of calcein enveloped in egg PCliposome bearing benzyl disulfide decreased from 62.7% to 49.2% and the mean hydrodynamic diameterof the liposome decreased from 276 nm to 206 nm when the egg PC to benzyl disulfide weight ratioincreased from 20:0 to 20:10. The liposomes bearing benzyl disulfide were multi-lamellar vesicles onTEM photos. It was confirmed by Raman spectroscopy that benzyl disulfide was loaded in the liposomalbilayer. The release degree of calcein enveloped in liposome incorporating benzyl disulfide wasinvestigated for 24 h at 25 C, 37 C, and 45 C, when the concentration of DL-dithiothreitol (DTT) was0 mM, 5 mM, 10 mM and 30 mM. At all the DTT concentrations tested, the release degree was moreextensive as the temperature was higher. At all the temperatures tested, the release degree became moreextensive as the DTT concentration increased. For example, the maximum release degree at 37 C ofcalcein loaded in liposome of which egg PC to benzyl disulfide weight ratio was 20:5 increased from 2.9%to 14.8% when DTT concentration increased from 0 mM to 10 mM. DTT could reduce benzyl disulfide tobenzyl thiols and the fragments of benzyl disulfide would re-orient due to its polarity change in theliposomal membrane, resulting in the membranefluctuation and the promoted release. At all thetemperatures and all the DTT concentrations tested, the release was more sensitive to the DTTconcentration as the benzyl disulfide content in liposome was higher, indicating that the reduction of thedisulfide compound was responsible for the promoted release.

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