The mold which could grow at alkaline pH and thermophilic condition and produced an extracellular proteolytic enzyme was identified as Trichoderma sp.. Trichoderma sp. CNU 301 exhibited the maximum production of proteolytic enzyme at 50℃ in the medi...
The mold which could grow at alkaline pH and thermophilic condition and produced an extracellular proteolytic enzyme was identified as Trichoderma sp.. Trichoderma sp. CNU 301 exhibited the maximum production of proteolytic enzyme at 50℃ in the medium composed of arabinose 1.5%, peptone 1.5% and FeSO₄0.2% (pH 9.0). The partial purified protease by precipitation with ammonium sulfate was most stable at pH 10 and at 60℃. The Trichoderma sp. CNU 301 produced an alkaline and thermostable protease.