微生物 蛋白質을 生産하기 爲한 메탄올 資化酵母에 關한 硏究

국문 초록 (Abstract)

Methanol을 利用하여 Single Cell Protein(SCP)을 生産하기 위한 基礎硏究로서 自然界에서 methanol資化酵母를 分離ㆍ同定하고 培養條件을 調査한 結果는 다음과 같다.<br/>
1. 本 methanol 資化酵母는 Candida boidinii로 同定되었고<br/>
2. 本 資化酵母의 最適 pH는 5.0이고 最適溫度는 28~30℃이었다.<br/>
3. 本 資化酵母는 最初 methanol濃度 2%의 培地에서 培養하면서 培養 36時間 後부터 每 12時間마다 0.5%의 methanol을 添加한 배지에서 가장 왕성한 生育을 보였다.<br/>
4. Thiamine(1000㎍/ℓ)과 biotin(10㎍/ℓ)을 添加했을때 生育이 促進되었다.<br/>
5. 最適 生育條件에서 0.268g/100㎖의 菌體量을 얻을 수 있었다.

다국어 초록 (Multilingual Abstract)

A methanol-assimilating yeast for the production of Single Cell Protein was isolated from the soil and identified. Methanol as a sole carbon source was used in this study. The strain was identified as Candida boidinii which grew best at the initial concentration of methanol at 2%, with the addition of 0.5% methanol at every 12 hours. The Single Cell Protein production was maximal after 72 hours of incubation at pH 5.0, 30℃. Thiamin and biotin were stimulated the growth of this yeast at the levels of 1000㎍/ℓ and 10㎍/ℓ, respectively.

목차 (Table of Contents)

• Abstract
  
• 序論
  
• 材料 및 方法
  
• 結果 및 考察
  
• 要約
  
• 參考文獻