한국인 구강 편평세포암종에서의 Ha-ras gene의 점돌연변이(point mutation)에 관한 연구 = Ha-ras gene point mutation in the oral squamous cell carcinoma of Korean

다국어 초록 (Multilingual Abstract)

The possible causes of oral squamous cell carcinoma include smoking, alcohol, viruses and chemicals. About 24 oncogenes involving the Ha-ras gene are known to be related to human and animal cancer. The ras gene point mutation varies among different tumors; for examples, Ha-ras gene is more frequent in human bladder carcinomas, Ki-ras gene is more frequent in lung and colon carcinomas, and N-ras gene in hematological tumors. The Ha-ras gene point mutation of oral SCC(squamous cell carcinoma) is variant from the race and geography, it appears to be frequent(about 35%) in Indian, but appears to be rare(about 3%) in Western.
This study was undertaken to investigate the rate of Ha-ras gene point mutation of the oral SCC in Korean. DNA extraction, PCR and SSCP, DNA sequencing of Ha-ras gene were done, and the point mutation was searched in the formaline fixed, paraffin-embeded tissues of the oral SCC from the 39 Korean patients (M:29, F:10).
Results were as follows:
1. The exon 1 of Ha-ras gene of 39 oral SCC was found in the 3 types(A, B, Ctypes) of band shift in the SSCP.
2. Each SSCP type A & B (37cases) of exon 1 has a normal DNA sequence, and no point mutation was detected.
3. Confirmation of SSCP type C of exon 1 was not possible presumably because of the artifact associated with DNA template purities.
4. The exon 2 of the Ha-ras gene in 39 ora SCC was found as a regular type of band shift in SSCP universally.
From these results, the point mutation of the Ha-ras gene of oral SCC in Korean proved to be rare, and other oncogenes than the Ha-ras gene may be related to carcinogenesis of oral SCC. Verifying the other oncogenes thought to be quite helpful in the treatment, prediagnosis, carcinogenesis of oral cancer.

목차 (Table of Contents)

• Abstract
• I. 서론
• II. 연구재료 및 방법
• III. 연구 결과
• IV. 총괄 및 고찰
• V. 결론
• 참고문헌