<i>Winogradskyella wandonensis</i> sp. nov., isolated from a tidal flat

다국어 초록 (Multilingual Abstract)

<P>A Gram-stain-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated WD-2-2<SUP>T</SUP>, was isolated from a tidal flat of Wando, an island of South Korea, and subjected to a polyphasic taxonomic analysis. Strain WD-2-2<SUP>T</SUP> grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WD-2-2<SUP>T</SUP> belonged to the genus <I>Winogradskyella</I>, clustering coherently with the type strain of <I>Winogradskyella litorisediminis</I>. Strain WD-2-2<SUP>T</SUP> exhibited 16S rRNA gene sequence similarity of 97.4 % to <I>W. litorisediminis</I> DPS-8<SUP>T</SUP> and 94.5–96.6 % to the type strains of the other species of the genus <I>Winogradskyella</I>. Strain WD-2-2<SUP>T</SUP> contained MK-6 as the predominant menaquinone and iso-C<SUB>15 : 1</SUB> G, iso-C<SUB>17 : 0</SUB> 3-OH and iso-C<SUB>15 : 0</SUB> as the major fatty acids. The major polar lipids detected in strain WD-2-2<SUP>T</SUP> were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content was 36.4 mol%, and DNA–DNA relatedness with <I>W. litorisediminis</I> DPS-8<SUP>T</SUP> was 13 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain WD-2-2<SUP>T</SUP> is separate from recognized species of the genus <I>Winogradskyella</I>. On the basis of the data presented, strain WD-2-2<SUP>T</SUP> is considered to represent a novel species of the genus <I>Winogradskyella</I>, for which the name <I>Winogradskyella wandonensis</I> sp. nov. is proposed. The type strain is WD-2-2<SUP>T</SUP> ( = KCTC 32579<SUP>T</SUP> = CECT 8445<SUP>T</SUP>).</P>