흰쥐 유양동 폐쇄술 후 시간에 따른 조직학적 변화 = Histologic Serial Changes of Obliterating Materials in the Rat Temporal Dorsal Bullae

다국어 초록 (Multilingual Abstract)

Background and Objectives：Mastoid obliteration surgery is a modern trend for otitis media either with or without cholesteatoma.
The aim of our study is to evaluate histologic changes and effectiveness over time resulting from different obliterating
materials and the existence of mucosa in the temporal dorsal bullae in rats. Materials and Method：Rats were divided into two
groups. One group had the mucosa removed and was treated with trichloroacetic acid (TCA). The other group with mucosa
remaining was untreated. The temporal dorsal bullae of the two groups of rats were obliterated with Mimix® (hydroxyapatite
cement), Regenafil® (demineralized bone matrix), cartilage chip, and bone chip. Three months and again six months after the
implantation, 5 animals in each group were examined. A histological study was performed to evaluate inflammation, new bone
formation, and mucocele formation within the bullae. Results：The group that had Mimix® implanted had a high inflammatory
reaction, low implanted material resorption and cyst formation. The group with Regenafil® implanted had high cyst formation
and more cyst formation with the passage of time. The group with bone chip implanted had high new bone formation, but also
high cyst formation. The group that had cartilage chip implanted had high new bone formation, low implanted material resorption
and low cyst formation. Conclusion：Cartilage chip is the only material that should be used in the air cell with mucosa
remaining. Demineralized bone matrix and bone chip should not be used in the air cell with mucosa remaining. Hydroxyapatite
cement should not be used due to severe inflammation.

참고문헌 (Reference)

1 구태우, "흰쥐 유양동 폐쇄술에 사용한 Hydroxyapatite Cement와 Demineralized Bone Matrix의 효과 및 조직병리학적 비교 연구" 대한이비인후과학회 51 (51): 777-782, 2008

2 Yung MW, "The use of hydroxyapatite granules in mastoid obliteration" 21 (21): 480-484, 1996

3 Leatherman BD, "The use of demineralized bone matrix for mastoid cavity obliteration" 25 (25): 22-26, 2004

4 Linthicum FH Jr, "The fate of mastoid obliteration tissue: A histopathological study" 112 (112): 1777-1781, 2002

5 Edwards JT, "Osteoinduction of human demineralized bone: Characterization in a rat model" (357) : 219-228, 1998

6 Mahendran S, "Mastoid obliteration with hydroxyapatite cement: The Ipswich experience" 25 (25): 19-21, 2004

7 Jang CH, "Mastoid obliteration using a hyaluronic acid gel to deliver a mesenchymal stem cells-loaded demineralized bone matrix: An experimental study" 72 (72): 1627-1632, 2008

8 Palva T, "Mastoid obliteration" (360) : 152-154, 1979

9 Lee WS, "Mastoid and epitympanic obliteration in canal wall up mastoidectomy for prevention of retraction pocket" 26 (26): 1107-1111, 2005

10 Kveton JF, "Indications for hydroxyapatite cement reconstruction in lateral skull base surgery" 16 (16): 465-469, 1995

11 Dornhoffer JL, "Impact on quality of life after mastoid obliteration" 118 (118): 1427-1432, 2008

12 Park KH, "Healing process of mastoid obliteration using cortical bone chips: Analysis of 90 cases" 38 (38): 345-352, 1995

13 Jang CH, "Evaluation of bioactive glass for mastoid obliteration: A guinea pig model" 21 (21): 651-655, 2007

14 Leatherman BD, "Demineralized bone matrix as an alternative for mastoid obliteration and posterior canal wall reconstruction: Results in an animal model" 22 (22): 731-736, 2001

15 Beales PH, "Complications following obliterative mastoid operation" 89 (89): 196-198, 1969

16 Jang CH, "Changes in external ear resonance after mastoidectomy: Open cavity mastoid versus obliterated mastoid cavity" 27 (27): 509-511, 2002

17 Dornhoffer J, "Canal wall reconstruction with Mimix hydroxyapatite cement: Results in an animal model and case study" 113 (113): 2123-2128, 2003

18 William HS, "Atlas of ear surgery. 4th ed" Mosby 1986

19 Takahashi S, "A morphological study on obliteration of the temporal dorsal bullae using hydroxyapatite granules" 17 (17): 197-199, 1996