대나무의 활성 성분 연구 = Isolation and Identification of Bioactive Compounds from Bamboos

다국어 초록 (Multilingual Abstract)

Phyllostachys bambusoides and Sasa borealis are two species belonging to bamboos, which are perennial grasses distributed widely in Asian countries including Korea, China and Japan. These plants have also been used in folklore medicine for the treatment of diabetes, arthritic pain, rheumatism and for their antidiuretic, antitussive, antipyretic activity.
The culms of Phyllostachys bambusoides and the leaves of Sasa borealis were extracted with 80% MeOH and the concd extracts were successively partitioned with n-hexane, CHCl3, EtOAc and n-BuOH, respectively. In the course of searching for bioactive compounds, six compounds were isolated from the n-hexane and CHCl3 extracts of bamboos, as guided by their DPPH free radical scavenging activities. The structures were determined as coniferaldehyde (1), vanillin (2), 2-hydroxy-4-methoxycinnamic acid (3), coniferyl alcohol (4), p-coumaric acid (5) and syringic acid (6). The structures of these compounds were identified by analysis of spectral data including 1D- and 2D-NMR spectra as well as by comparison of their data with the published values. Antioxidant activities of compounds 1-6 were evaluated for their DPPH radical scavenging effects, and compounds 5 and 6 showed similar activities compared with vitamin C.

국문 초록 (Abstract)

대나무는 다년생 풀로서 전 세계적으로 널리 분포되어 있으며, 그 중 한국 남부지방에는 Phyllostachys와 Sasa 속이 자생하고 있다. 대나무는 예로부터 민간에서 약재로 사용하였으며, 특히 전통적으로 항산화 능력과 관계가 깊은 병의 치료에 이용되었다. 이에 본 연구에서는 왕대 줄기와 조릿대 잎에서 항산화 활성이 있는 물질을 탐색하였다.
왕대줄기와 조릿대 잎을 80% MeOH로 추출한 후, n-hexane, CHCl3, EtOAc, n-BuOH과 aqueous 분획으로 나누고, 항산화 활성을 나타낸 분획에 대해 column chromatography를 실시하여 6종의 화합물을 분리, 정제하였다. NMR, UV, IR, MS 등 각종 분광분석법을 통하여 분석한 결과, 이 화합물들의 화학구조를 coniferaldehyde (1), vanillin (2), 2-hydroxy-4-methoxycinnamic acid (3), coniferyl alcohol (4), p-coumaric acid (5) 및 syringic acid (6)로 동정하였다. 이 중에서 2-hydroxy-4-methoxycinnamic acid (3)와 coniferyl alcohol (4)은 대나무에서 처음으로, 그리고 syringic acid (6)는 조릿대에서 처음으로 분리되었다. 6종 화합물의 항산화 활성은 DPPH를 이용한 라디칼 소거능으로 측정하였으며, 이 중에서 p-coumaric acid (5)와 syringic acid (6)가 vitamin C와 비슷한 항산화 활성을 나타냈다.

목차 (Table of Contents)

• Abstract (in English) = 1
• INTRODUCTION = 2
• EXPERIMENTAL = 6
• 1. General experimental procedures = 6
• 2. Plant materials = 6
• 3. Extraction and bioassay-directed fractionation = 7
• 4. Antioxidant activity evaluation: Scavenging effect on DPPH radical = 11
• RESULTS AND DISCUSSION = 15
• 1. Structure elucidation of isolated compounds = 15
• 2. Antioxidant activity of compounds 1-6 = 19
• REFERENCES = 47
• Abstract (in Korean) = 58
• ACKNOWLEDGMENTS = 59