소 설사병 바이러스 구조단백질 E2의 대장균 발현

다국어 초록 (Multilingual Abstract)

The gene corresponding envelope(E2, C-terminal of El and E2) of bovine viral diarrhea virus (BVDV) was expressed in downstream from the malE gene of E. coli, resulting in the expression of fusion protein with maltose-binding protein(MBP). The expressed MBP-E2 fusion protein was affinity purified for the production of antiserum and the expression of E2 was identified by immunoblotting with antiserum against MBP E2 fusion protein. Although the protein band with molecular weight above 88 kDa was identified, the cell expressing C-terminal of El and E2 showed the degradation of expressed fusion protein. However, low molecular weight protein bands including MBP were frequently detected in immunoblotting assay, thus, indicating that C-terminal of El can play a role of signal peptide after translation in similar pattern of eukaryotic cell.