성견 치주질환시 치은 열구액내의 μ-glucuronidase에 관한 실험적 연구 = An Experimental Study on μ-glucuronidase in Gingival Crevicular Fluid of Doge with Periodontal Disease

다국어 초록 (Multilingual Abstract)

Analysis of an enzyme in gingival crevicular fluid is considered as a valuable diagnostic method for periodontal inflammation. The relationship between enzyme activity and clinical parameters can also be employed to evaluate the periodontal status. The object of this study was to present the diagnostic value of the β-glucuronidase in gingival crevicular fluid during periodontal breakdown. This study was examined with 4 dogs weighed 13-15㎏, being ligated with wire at third and forth premolars of both jaws while contralateral teeth were brushed three times a week as a control. Two months after the ligation, when periodontitis was induced, the gingival crevicular fluid was collected every one week to be calculated. The following results were obtained by comparison of enzyme activity with clinical parameters : sulcus bleeding index, plaque index, pocket depth.
1. The optical density of β-glucuronidase activity were 0.180±0.060 at the experimental sites and 0.128±0.030 at the control sites (p<0.01).
2. The relationships between clinical indices and periotron units, and β-glucuronidase activity were statistically significant (p<0.01), while correlation between β-glucuronidase activity and pocket depth was less significant(p<0.05).
3. As the grade of sulcus bleeding index was increased, the periotron units and β-glucuronidase activity were increased significantly (periotron unit ; Grade Ⅲ p<0.01, Ⅳ : p<0.01., β-glucuronidase activity ; Grade Ⅳ : p<0.05).
4. As the grade of pocket depth was increased, periotron units increased significantly (p<0.05).