Light dependent expression of pea chloroplast fructose-1,6-bisphosphatase

목차 (Table of Contents)

• CONTENTS
• Intorduction = 1
• Materials and Methods
• Materials = 5
• Growth conditions = 6
• Purification of pea chloroplast fructose-1,6-bisphosphatase = 6
• SDS polyacrylamide gel electrophoresis = 7
• Enzyme assay system = 8
• Preparation of total pea RNA = 8
• Isolation of poly(A) RNA = 9
• Preparation of antisera = 10
• Immunoblot analysis = 11
• Northern hybridization = 11
• Bacterial strains = 12
• Culture media = 12
• Phage DNA isolation = 13
• Subcloning = 14
• Transformation = 14
• Mini-scale isolation of plasmid DNA = 15
• Large-scale isolation of plasmid DNA = 15
• cDNA library construction = 17
• Immunological screening of pea leave cDNA library = 18
• DNA sequencing = 18
• Result
• Preparation of anti-chloroplast fructose-1,6-bisphosphatase antibody = 21
• Effect of light or dark treated on chloroplast fructose-1,6-bisphosphatase = 21
• Effect of red and far-red light on chloroplast fructose-1,6-bisphosphatase = 22
• Chloroplast frucose-1,6-bisphosphatase activities of red or far-red light treated etiolated pea seedlings = 23
• Tissue-specific expression chloroplast fructose-1,6-bisphosphatase = 24
• Time course for white light induced increase in chloroplast fructose-1,6-bisphosphatese mRNA levels = 24
• Isolation of fructose-1,6-bishposphatese cDNA clone and subcloning of A55, A53 = 25
• Nucleotide sequence analysis of pJH552 and himology search = 25
• Discussion = 27
• Figure legends = 42
• References = 45
• Abstract in Korean = 49