Effects of $tat^-$ and $ssd^-$ Mutation on the Expression of a Recombinant trp Plasmid and Tryptophan Production in Klebsiella pnenmoniae = Klebsiella pneumoniae의 L-serine deaminase와 tryptophan aminotransferase 결실 변이가 E. coli trp operon 발현에 미치는 영향

국문 초록 (Abstract)

트립토판 생산성 증대를 위한 균주 개발의 일환으로 숙주 박테리아인 Klebsiella pneumoniae의 5-methyltryptophan 내성주(5-$MT^r$), tryptophan aminotransferase 결손주($tat^-$) 및 L-serine deaminase의 결손주($ssd^-$)를 얻고, 또한 이들 변이가 증폭된 재조합 E. coli trp operon ($R_6K::Mu-trpL({\Delta}tt)trpE^{FBR}$)의 발현에 어떻게 영향을 미치는지 조사하였다. K. pneumoniae에서 5-$MT^r$, $tat^-$, $ssd^-$ 돌연변이는 재초합 E. coli trp operon의 발현을 증가시켰으며, trp operon의 최종산물인 트립토판의 생산성도 증가시켰다.

다국어 초록 (Multilingual Abstract)

As a part of the host development for a amplified recombinant E. coli trp operon ($R_6K::Mu-trpL({\Delta}att)trpE^{FBR}$) for the production of tryptophan, the 5-methyltryptophan-resistan mutation (5-$MT^r$), tryptophan aminotransferase-deficient mutation ($tat^-$) and L-serine deaminase-deficient mutation ($ssd^-$) were introduced into the K. pneumoniae host cell and their effects on the expression of the try operon and production of tryptophan were examined. The 5-$MT^r$ mutation derepressed the expression of the recombinant E. coli trp operon and both $tat^-$ and $ssd^-$ mutation increased the activities of anthranilate synthetase and tryptophan synthetase as well as the producation of tryptophan in K. pneumoniae.