Phenobarbital 前處置가 Halothane 麻醉時 肝病變에 미치는 影響에 關한 病理組織學的 硏究 = Histopathological Studies on Effects of Phenobarbital Pretreatment on the Response of Rat Liver to Halothane Anesthesia

다국어 초록 (Multilingual Abstract)

Since halothane, as a new potent, nonexplosive, volatile agent, was introduced in 1956, many case reports of liver necrosis following halothane anesthesia have raised to possibility that the agent may damage the liver under certain circumstances.
This study was carried out to investigate the histopathological effects of phenobarbital pretreated halothane anesthesia to the rat liver cells.
Sprague-Dawley rats (male and female) were pretreated with phenobarbital; PBT(100 mg/kg/ day) for 3 days.
All animals in each experimental group (saline-control, saline-anesthetized, PBT-control, and PBT-anesthetized) were fasted for 15 to 16 hours prior to each anesthesia period.
PBT and Saline-pretreated animals were anesthetized together for 3 hours one to two times at 3 day intervals in a single chamber from a calibrated vaporizer.
Stage III anesthesia was introduced with 1% halothane in oxygen and maintained with 0.8% to 1% halothane.
Controls received the equivalent amount of saline solution.
Each animal was sacrificed immediately, at 6, 12, 18, 24 and 48 hours after anesthesia respectively.
The histopathological changes in the liver of the control and experimental groups were noted as follows;
1. Vacuolar degeneration of the hepatic cells of PBT pretreated rats showed more marked degree than the control.
In repeated exposure of animals to the anesthetic at 3 day intervals aggravates the lesioned in control and PBT pretreated rats.
2. In first exposure to the anesthetic, fatty changes of the hepatic cells in PBT pretrea rats showed more marked in degree than the control.
In repeated exposure of rats aggravates the lesions in control and PBT pretreated rats, and that of PBT pretreated rats was similar to the control.
3. The marked necrotic changes of hepatic cells were present in the PBT pretreated rats but those of the control were rarely observed.
Repeated exposure of rats aggravate the lesion.