Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb Mining = Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb Mining

다국어 초록 (Multilingual Abstract)

Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this study was to investigate the colonization and molecular diversity of AMF associated with the Sb accumulator ramie (Boehmeria nivea) growing in Sb-contaminated soils. Four Sb mine spoils and one adjacent reference area were selected from Xikuangshan in southern China. PCR-DGGE was used to analyze the AMF community composition in ramie roots. Morphological identification was also used to analyze the species in the rhizosphere soil of ramie. Results obtained showed that mycorrhizal symbiosis was established successfully even in the most heavily polluted sites. From the unpolluted site Ref to the highest polluted site T4, the spore numbers and AMF diversity increased at first and then decreased. Colonization increased consistently with the increasing Sb concentrations in the soil. A total of 14 species were identified by morphological analysis. From the total number of species, 4 (29%) belonged to Glomus, 2 (14%) belonged to Acaulospora, 2 (14%) belonged to Funneliformis, 1 (7%) belonged to Claroideoglomus, 1 (7%) belonged to Gigaspora, 1 (7%) belonged to Paraglomus, 1 (7%) belonging to Rhizophagus, 1 (7%) belonging to Sclervocystis, and 1 (7%) belonged to Scutellospora. Some AMF sequences were present even in the most polluted site. Morphological identification and phylogenetic analysis both revealed that most species were affiliated with Glomus, suggesting that Glomus was the dominant genus in this AMF community. This study demonstrated that ramie associated with AMF may have great potential for remediation of Sb-contaminated soils.

참고문헌 (Reference)

1 Vogel-Mikuš K, "Zn, Cd and Pb accumulation and arbuscular mycorrhizal colonisation of pennycress Thlaspi praecox Wulf. (Brassicaceae) from the vicinity of a lead mine and smelter in Slovenia" 133 : 233-242, 2005

2 Yang RY, "Variation in community structure of arbuscular mycorrhizal fungi associated with a Cu tolerant plant - Elsholtzia splendens" 44 : 191-197, 2010

3 Miransari M, "Using arbuscular mycorrhiza to reduce the stressful effects of soil compaction on wheat (Triticum aestivum L .) growth" 40 : 1197-1206, 2008

4 Kabata A, "Trace Elements in Soils and Plants" CRC Press 2001

5 United States Environmental Protection Agency, "Toxics release inventory"

6 She W, "Tolerance to cadmium in ramie (Boehmeria nivea) genotypes and its evaluation indicators" 37 : 348-354, 2011

7 Hildebrandt U, "The zinc violet and its colonization by arbuscular mycorrhizal fungi" 154 : 709-717, 1999

8 Feng RW, "The uptake and detoxification of antimony by plants:a review" 96 : 28-34, 2013

9 Peng JF, "The remediation of heavy metals contaminated sediment" 161 : 633-640, 2009

10 Boyd RS, "The defense hypothesis of elemental hyperaccumulation: status, challenges and new directions" 293 : 153-176, 2007

11 Wilson SC, "The chemistry and behaviour of antimony in the soil environment with comparisons to arsenic: a critical review" 158 : 1169-1181, 2010

12 MEMSC, "The background values of soil elements in China" Meterological Press 1990

13 Cornejo P, "Temporal temperature gradient gel electrophoresis (TTGE)as a tool for the characterization of arbuscular mycorrhizal fungi" 241 : 265-270, 2004

14 Sonjak S, "Temporal temperature gradient gel electrophoresis (TTGE) analysis of arbuscular mycorrhizal fungi associated with selected plants from saline and metal polluted environments" 314 : 25-34, 2009

15 Punaminiya P, "Symbiotic role of Glomus mosseae in phytoextraction of lead in vetiver grass Chrysopogon zizanioides ( L.)" 177 : 465-474, 2010

16 Leung HM, "Survival strategies of plants associated with arbuscular mycorrhizal fungi on toxic mine tailings" 66 : 905-915, 2007

17 Wang X, "Subcellular distribution and chemical forms of cadmium in Bechmeria nivea (L.) Gaud" 62 : 389-395, 2008

18 Gerdemann JW, "Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting" 46 : 235-244, 1963

19 Simon L, "Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots" 58 : 291-295, 1992

20 Khan A., "Role of soil microbes in the rhizospheres of plants growing on trace metal contaminated soils in phytoremediation" 18 : 355-364, 2005

21 Amir H, "Relationships between Ni-hyperaccumulation and mycorrhizal status of different endemic plant species from New Caledonian ultramafic soils" 293 : 23-35, 2007

22 Gaur A, "Prospects of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils" 86 : 528-534, 2004

23 Muyzer G, "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction genes coding for 16S rRNA" 59 : 695-700, 1993

24 Helgason T, "Ploughing up the wood-wide web?" 394 : 431-, 1998

25 Reeves RD, "Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment" John Wiley & Sons Inc 193-229, 2000

26 Perrier N, "Occurrence of mycorrhizal symbioses in the metal-rich lateritic soils of the Koniambo Massif, New Caledonia" 16 : 449-458, 2006

27 Al Agely A, "Mycorrhizae increase arsenic uptake by the hyperaccumulator Chinese brake fern (Pteris vittata L.)" 34 : 2181-2186, 2005

28 Barea JM, "Mycorrhiza Structure, Function, Molecular Biology and Biotechnology" Springer-Verlag 521-559, 1995

29 Daniell TJ, "Molecular diversity of arbuscular mycorrhizal fungi colonizing arable crops" 36 : 203-209, 2001

30 Hammel W, "Mobility of antimony in soil and its availability to plants" 41 : 1791-1798, 2000

31 Lu RK, "Method of soil chemistry analysis" Chinese Agricultural Science and Technology Publishing House 146-149, 2000

32 Alford ÉR, "Metallophytes - a view from the rhizosphere" 337 : 33-50, 2010

33 Wu FY, "Metal accumulation and arbuscular mycorrhizal status in metallicolous and non metallicolous populations of Pteris vittata L. and Sedum alfredii Hance" 226 : 1363-1378, 2007

34 Tamura K, "MEGA4:molecular evolutionary genetics analysis (MEGA) software version 4.0" 24 : 1596-1599, 2007

35 Liu FH, "Introduction to the wild resources of the genus Boehmeria Jacq. in China" 50 : 793-797, 2003

36 Wu FY, "Intraspecific differences of arbuscular mycorrhizal fungi in their impacts on arsenic accumulation by Pteris vittata L" 76 : 1258-1264, 2009

37 Leung HM, "Interactions of mycorrhizal fungi with Pteris vittata (As hyperaccumulator)in As-contaminated soils" 139 : 1-8, 2006

38 Miransari M., "Interactions between arbuscular mycorrhizal fungi and soil bacteria" 89 : 917-930, 2011

39 Liu Y, "Influence of the arbuscular mycorrhizal fungus Glomus mosseae on uptake of arsenate by the As hyperaccumulator fern Pteris vittata L" 15 : 187-192, 2005

40 Ku WZ, "Heavy metal pollution in soils and characteristics of heavy metal accumulation of dominant plants in antimony mine area" 10 : 3774-3780, 2012

41 Schwarzott D, "Glomus, the largest genus of the arbuscular mycorrhizal fungi (Glomales), is nonmonophyletic" 21 : 190-197, 2001

42 Schüßler A, "Glomeromycota: a species list with new families and new genera"

43 Altschul SF, "Gapped BLAST and PSI-BLAST:a new generation of protein database search programs" 25 : 3389-3402, 1997

44 Niu ZC, "Effects of lead and zinc in the soil on the distribution of arbuscular mycorrhizal fungi" 27 : 1233-1238, 2007

45 Wang FY, "Effects of arbuscular mycorrhizal inoculation on the growth of Elsholtzia splendens and Zea mays and the activities of phosphatase and urease in a multi-metal-contaminated soil under unsterilized conditions" 31 : 110-119, 2006

46 Ricken B, "Effects of arbuscular mycorrhizal fungi (AMF) on heavy metal tolerance of alfalfa (Medicago sativa L.) and o at (Avena sativa L.) on a sewage-sludge treated soil" 159 : 189-194, 1996

47 Leyval C, "Effect of heavy metal pollution on mycorrhizal colonization and function:physiological, ecological and applied aspects" 7 : 139-153, 1997

48 Solís DFA, "Effect of arbuscular mycorrhizal fungi on plant biomass and the rhizosphere microbial community structure of mesquite grown in acidic lead/zinc mine tailings" 409 : 1009-1016, 2011

49 Baker AJM, "Ecology of Industrial Pollution" Cambridge University Press 7-40, 2010

50 Okkenhaug G, "Distribution, speciation and availability of antimony (Sb) in soils and terrestrial plants from an active Sb mining area" 159 : 2427-2434, 2011

51 He MC, "Distribution and phytoavailability of antimony at an antimony mining and smelting area, Hunan, China" 29 : 209-219, 2007

52 Göhre V, "Contribution of the arbuscular mycorrhizal symbiosis to heavy metal phytoremediation" 223 : 1115-1122, 2006

53 Yang B, "Constitutional tolerance to heavy metals of a fiber crop, ramie (Boehmeria nivea), and its potential usage" 158 : 551-558, 2010

54 Zarei M, "Community structure of arbuscular mycorrhizal fungi associated to Veronica rechingeri at the Anguran zinc and lead mining region" 156 : 1277-1283, 2008

55 Regvar M, "Colonization of pennycresses (Thlaspi spp.) of the Brassicaceae by arbuscular mycorrhizal fungi" 160 : 615-626, 2003

56 Vogel-Mikuš K, "Colonisation of a Zn, Cd and Pb hyperaccumulator Thlaspi praecox Wulfen with indigenous arbuscular mycorrhizal fungal mixture induces changes in heavy metal and nutrient uptake" 139 : 362-371, 2006

57 Brundrett MC, "Coevolution of roots and mycorrhizas of land plants" 154 : 275-304, 2002

58 Pongrac P, "Changes in elemental uptake and arbuscular mycorrhizal colonization during the life cycle of Thlaspi praecox Wulfen" 69 : 1602-1609, 2007

59 Luo HF, "Assessment of the bacterial diversity in fenvalerate-treated soil" 20 : 509-515, 2004

60 Vallino M, "Assessment of arbuscular mycorrhizal fungal diversity in roots of Solidago gigantea growing in a polluted soil in Northern Italy" 8 : 971-983, 2006

61 Gebel T., "Arsenic and antimony: comparative approach on mechanistic toxicology" 107 : 131-144, 1997

62 Zarei M, "Arbuscular mycorrhizal abundance in contaminated soils around a zinc and lead deposit" 44 : 381-391, 2008

63 Trotta A, "Arbuscular mycorrhizae increase the arsenic translocation factor in the As hyperaccumulating fern Pteris vittata L" 65 : 74-81, 2006

64 Dong Y, "Arbuscular mycorrhiza enhanced arsenic resistance of both white clover (Trifolium repens Linn.) and ryegrass (Lolium perenne L.) plants in an arsenic-contaminated soil" 155 : 174-181, 2008

65 Index Mundi, "Antimony: world mine production, by country"

66 He MC, "Antimony pollution in China" 421 (421): 41-50, 2012

67 Tschan M, "Antimony in the soil–plant system – a review" 6 : 106-115, 2009

68 Filella M, "Antimony in the environment: a review focused on natural waters: I. Occurrence" 57 : 125-176, 2002

69 Flynn HC, "Antimony bioavailability in mine soils" 124 : 93-100, 2003

70 Pan XL, "Antimony accumulation, growth performance, antioxidant defense system and photosynthesis of Zea mays in response to antimony pollution in soil" 215 : 517-523, 2011

71 Feng RW, "Antimony accumulation and antioxidative responses in four fern plants" 317 : 93-101, 2009

72 Redecker D, "An evidence-based consensus for the classification of arbuscular mycorrhizal fungi (Glomeromycota)" 23 : 515-531, 2013

73 Fusconi A, "Abiotic stress responses in plants. Metabolism, Productivity and Sustainability" Springer 197-214, 2012

74 Schwarzott D, "A simple and reliable method for SSU rRNA gene DNA extraction, amplification and cloning from single AM fungal spores" 10 : 203-207, 2001

75 McGonigle TP, "A new method which gives an objective measure of colonization of roots by vesicular arbuscular mycorrhizal fungi" 115 : 495-501, 1990