가열온도, 가열시간 및 부형제의 첨가량이 도축 반추위 내용물의 자일란, 셀룰로오스 및 전분 분해효소 활성에 미치는 영향 = Effects of Temperature and Time for Heating and Filler Content on the Activities of Xylanase, Cellulase and Amylase in Slaughterhouse Rumen Content

국문 초록 (Abstract)

본 연구는 도축장에서 폐기되는 도축 반추위 내용물을 사일리지 혹은 TMR (total mixed ration) 사료 첨가용 효소제로 개발하기 위한 목적으로 수행되었다. 도축 반추위 내용물에는 상당한 수분이 함유되어 있어, 적절한 건조과정을 거치지 않고서는 그 활용이 원활하지 않다. 그러나 효소는 열에 민감한 단백질로 구성되어 있기 때문에 적절한 건조 조건의 개발이 필요하다. 본 연구에서는 통계적 방법을 이용하여 가열온도(60, 75, $90^{\circ}C$), 가열시간(12, 30, 48 시간) 및 부형제의 비율(12, 22.5, 33%)이 도축 반추위 내용물에 함유된 다양한 효소활성에 미치는 효과를 분석하였다. 총 3가지 효소, xylan 분해효소, 셀룰로오스 분해효소 및 전분 분해효소를 검토하였고, 각 효소활성들에 대한 각 요인들의 효과가 매우 다양하게 나타났다(p<0.05). 셀룰로오스 분해효소와 전분 분해효소의 활성은 가열온도가 증가함에 따라 감소하였으며(p<0.05), xylan 분해해소는 열에 대한 안전성이 다른 효소들에 비하여 우수하였다. 자일란, 셀룰로오스, 전분 분해효소를 증가시키는 적정 부형제의 비율은 22.5, 12 그리고 33%로 나타났다. 비록 3가지 효소들의 제형화에 있어 공통적으로 적용될 수 있는 최적점을 도출하지는 못하였으나, 본 연구결과에서 얻어진 효소들의 반응 값들을 이용하여 목적하는 효소에 따라서 다양한 방법으로 적용이 가능할 것으로 판단된다.

다국어 초록 (Multilingual Abstract)

This study was conducted in order to develop slaughterhouse rumen content (SRC) as a potential feed additive. The moisture content of SRC can reach 80%, and therefore an appropriate dewatering process is required before it can be used. In this study, the effects of heating temperature, heating time, and filler content during the dewatering process on the activity of various enzymes in SRC were investigated. The Box-Behnken experimental design was employed, involving a total of 45 experimental runs, consisting of three variables (heating time, heating temperature, and filler content) with three levels per variable (12, 30 and 48 hr; 60, 75 and $90^{\circ}C$; 12, 22.5 and 33% for heating time, heating temperature, and filler content, respectively). For enzyme activities, xylanase, cellulase, and amylase were examined, and the results were subjected to an analysis of variance. Heating time, heating temperature and filler content had significant effects on the activity of each enzyme (p<0.05). Cellulase and amylase activities decreased (p<0.05) at elevated heating temperatures, whereas xylanase was reasonably stable around $90^{\circ}C$. The activities of all enzymes decreased (p<0.05) with increased heating time. Optimum filler contents for xylanase, cellulase, and amylase activities were 22.5, 12 and 33%, respectively. However, optimum conditions for all variables that simultaneously maximize the activity of all three enzymes could not be ascertained in this study. Nevertheless, the results from the current study can be useful as basic information for the development of SRC as a feed additive enriched with improved major enzymes for livestock feed digestion.

참고문헌 (Reference)

1 손호진, "탄소원의 종류가 반추위 혐기 곰팡이 Neocallimastix sp. NLRI-3의 섬유소 분해효소 활력에 미치는 영향 비교" 한국동물자원과학회 48 (48): 415-424, 2006

2 Kim, B, "Study on the development of Aspergillus oryzae culture with rumen contents from slaughterhouse" 42 : 85-92, 2000

3 Box, G.E.P, "Some new three level designs for the study of quantitative variables" 2 : 455-475, 1960

4 Carpenter, J.F, "Separation of freezing- and drying-induced denaturation of lyophilized proteins using stress-specific stabilization: I. Enzyme activity and calorimetric studies" 303 : 456-464, 1993

5 Santra, A, "Rumen enzyme profile and fermentation characteristics in sheep as affected by treatment with sodium lauryl sulfate as defaunating agent and presence of ciliate protozoa" 67 : 126-137, 2007

6 Schingoethe, D. J, "Response of lactating dairy cows to a cellulase and xylanase enzyme mixture applied to forages at the time of feeding" 82 : 996-1003, 1999

7 Ruf, E. W, "Observations upon an unidentified factor in feedstuffs stimulatory to cellulose digestion in the rumen and improved liveweight gains in lambs" 12 : 731-739, 1953

8 Jovanović, M, "Nutritive value of rumen contents for monogastric animals" 2 : 351-360, 1977

9 Weiss, W.P, "Milk production and nutrient digestibility by dairy cows when fed exogenous amylase with coarsely ground dry corn" 94 : 2492-2499, 2011

10 Minitab Inc, "Meet Minitab 16"

11 Holtshausen, L, "Improved milk production efficiency in early lactation dairy cattle with dietary addition of a developmental fibrolytic enzyme additive" 94 : 899-907, 2011

12 Salinas-Chavira, J, "Growth performance and carcass characteristics of feedlot lambs fed diets with pig manure and rumen contents" 6 : 505-508, 2007

13 Bryant, M.P, "Factors necessary for the growth of Bacteroides succinogenes in the volatile acid fraction of rumen fluid" 38 : 340-350, 1955

14 Yang, W.Z, "Effectsof an enzyme feed additive on extent of digestion and milk production of lactating dairy cows" 82 : 391-403, 1999

15 Adeniji, A.A, "Effects of replacing maize with enzyme-supplemented bovine rumen content in the diets of pullet chicks" 6 : 814-817, 2007

16 Arriola, K.G, "Effect of fibrolytic enzyme application to low- and high-concentrate diets on the performance of lactating dairy cattle" 94 : 832-841, 2011

17 Bhatta, R, "Effect of feeding complete feed block containing Prosopis cineraria leaves and polyethylene glycol (PEG)-6000 on nutrient intake, its utilization, rumen fermentation pattern and rumen enzyme profile in kids" 85 : 1788-1794, 2005

18 Titi, H, "Effect of feeding cellulase enzyme on productive responses of pregnant and lactating ewes and goats" 52 : 137-143, 2004

19 Gencoglu, H, "Effect of feeding a reduced-starch diet with or without amylase addition on lactation performance in dairy cows" 93 : 723-732, 2010

20 Rincon, F.G.R, "Dried ruminal contents as a substitute for alfalfa hay in growing-finishing diets for feedlot cattle" 9 : 1526-1530, 2010

21 Shrestha, K, "Changes in microbial and nutrient composition associated with rumen content compost incubation" 102 : 3848-3854, 2011

22 Forsberg, C.W, "Biotechnology and Nutrition" Butterworth Heinmann Stoneham 107-147, 1992

23 Klingerman, C.M, "An evaluation of exogenous enzymes with amylolytic activity for dairy cows" 92 : 1050-1059, 2009