포도 뿌리혹병 방제를 위한 길항균 선발 = Isolation of Antagonists for Biological Control of Crown Gall Disease on Grape

다국어 초록 (Multilingual Abstract)

Previous study showed that crown gall disease is very severe on the tetraploid cultivars of grape. One of the promising control measures of the disease is biological control using a antagonist against pathogen, Agrobacterium vitis. Total 215 bacteria and 21 putative agrobacteria were isolated from galls of grapevine and a Korean wild grape, Vitis amurensis respectively. Antagonistic ability of the isolates was checked by growth inhibition test of pathogens, A. tumefaciens and A. vitis on agar and on tamato seedling by co-inoculation of the isolates and pathogen. Two selected antagonists, isolate 197 and Va 21 strongly suppressed gall formation of A. vitis K306 on grapevine when they were co-inoculated with pathogen K306. Both gall size and rate of gall formation were significantly lower on grapevines inoculated of 197-K306(9:1) or Va 21-K306(9:1) mixture than on them inoculated of just K306. Antagonistic ability of the two isolates was similar to F2/5, which is known as a strongest antagonist against A. vitis so far. Further field study for biological control of crown gall disease on grapevine by the two isolates is needed.