Pentoxifylline과 단당류 fructose와 galactose가 정자운동에 미치는 영향 = Effects of Pentoxifylline and Monosaccharide fructose and galactose on Sperm Motility

다국어 초록 (Multilingual Abstract)

Sperm motility is an important factor in fertilization and pregnancy. The sperm viability of commercial cow frozen semen and boar liquid semen tends to decrease after freezing compared with before freezing and as the sperm storage time increases, respectively. Before in vitro fertilization or intrauterine insemination, pentoxifylline as a cell signaling pathway factor can be used to improve sperm motility. Pentoxifylline inhibits phosphodiesterase activity and continuously increases intracellular cAMP levels. This increased cAMP promotes the activity of enzymes involved in the production of ATP required for sperm motility. Therefore, pentoxifylline is thought to be able to improve sperm motility through cell signaling pathways. In addition, monosaccharides like fructose and galactose as the energy sources to produce ATP is thought to be able to improve sperm motility. In other words, pentoxifylline(PTX) and monosaccharide when added to media for sperm incubation is thought to be able to improve sperm motility. In order to propose a culture condition to improve the motility of bull and boar sperm, this study investigated the effect of pentoxifylline and monosaccharides fructose and galactose on sperm motility. Cow frozen semen and boar liquid semen was used to analyze the effect of PTX and monosaccharide on sperm motility with sperm motility index and computer assisted sperm analyser(CASA) method. The effect of PTX treatment on cow frozen semen was investigated, 5mM PTX significantly increased sperm motility(MOT) compared to control. The effect of monosaccharide on sperm motility was investigated, 10mM fructose increased sperm motility VAP, ALH and BCF, but galactose did not affect sperm motility. In addition, the effect of PTX and monosaccharide combined treatment on bull sperm motility was investigated, they did not increased sperm motility. The effect of PTX treatment on boar liquid semen was investigated, 5mM PTX significantly increased the sperm motility compared to other treatments, especially at the culture for 40 minutes. The effect of monosaccharide on sperm motility was investigated, 10mM fructose significantly increased sperm motility VAP and 20mM fructose significantly increased sperm motility VCL, compared to the 5mM frcutose, respectively. However, 20mM galactose significantly increased sperm motility VSL compare to control, especially at the cultures for 40minutes and 50minutes. In addition, the effect of PTX and monosaccharide combined treatment on boar sperm motility was investigated, Of all treatments, sperm motility VCL and VSL were increased only in 20mM PTX and 10mM galactose combined treatment. In conclusion, PTX inproved sperm movement in cow frozen semen and boar liquid semen. Especially, PTX 5mM may be a optimal concentration for the sperm motility. On the other hand, 10mM fructose or 20mM galactose may increase effectively the sperm motility in boar liquid semen. The combined treatment of 20mM PTX and 10mM galactose can improve the sperm motility in the boar semen. Therefore, in order to improve the movement of sperm in frozen and liquid semen, it is recommended to add 5mM PTX to the media or diluent at 40 minutes before use.

목차 (Table of Contents)

• Ⅰ서론 5
• Ⅱ재료 및 방법 6
• 1. 정자 준비 6
• 2. 실험 설계 6
• 3. 실험방법 7
• 1) PTX 효과분석 7
• ⑴ 활력지수법 분석 7
• ⑵ Computer assisted semen analysis (CASA) 분석 7
• 2) 단당류 효과 분석 8
• 3) PTX와 단당류 공효과 분석 9
• 4. 통계분석 9
• Ⅲ. 결과 10
• 1. PTX가 정자 운동성에 미치는 효과 10
• 1) PTX 농도의 효과 10
• 2) PTX 5mM 효과 11
• ⑵ Computer assisted semen analysis (CASA) 분석 11
• 2. 단당류의 효과분석 12
• 1) Fructose의 효과 12
• 2) Galactose의 효과 18
• 3. PTX와 단당류 공효과분석 24
• 1)PTX와 Fructose 공효과 24
• 2)PTX와 Galactose 공효과 30
• Ⅳ. 고찰 36
• 연구II 37
• Ⅰ. 서론 38
• Ⅱ. 재료 및 방법 39
• 1. 정자 준비 39
• 2. 실험 설계 39
• 3. 실험방법 40
• 1) PTX 효과분석 40
• ⑴ Computer assisted semen analysis (CASA) 분석 40
• 2) 단당류 효과 분석 41
• 3) PTX와 단당류 공효과 분석 42
• 4. 통계분석 42
• Ⅲ. 결과 43
• 1. PTX가 정자의 운동성에 미치는 효과 43
• 1) PTX 농도의 효과 43
• 2. 단당류가 정자의 운동성에 미치는 효과 50
• 1) Fructose의 효과 50
• 2) Galactose의 효과 56
• 3. PTX와 단당류의 공효과 분석 62
• 1) PTX와 Fructose의 공효과 62
• ⑴ PTX 5mM와 Fructose 공효과 63
• ⑵ PTX 20mM와 Fructose 공효과 69
• 2) PTX와 Galactose의 공효과 75
• ⑴ PTX 5mM와 Galactose의 공효과 76
• ⑵ PTX20mM와 Galactose의 공효과 82
• 3) PTX에서 Fructose와 Galactose 효과 88
• ⑴ Motility (MOT) 비교 88
• ⑵ Average Path Velocity (VAP) 비교 95
• ⑶ Curvilinear Velocity (VCL) 비교 101
• ⑷ Straight-line Velocity (VSL) 비교 107
• ⑸ Amplitude of lateral Head (ALH) 비교 113
• ⑹ Beat Cross Frequency (BCF) 비교 119
• Ⅳ. 고찰 125
• 종합고찰 128
• 참고문헌 132abstract 138