Cortisone acetate 投與한 마우스 組織에서의 免疫組織化學的方法에 依한 Toxoplasma 抗原 檢出에 關한 硏究

다국어 초록 (Multilingual Abstract)

This study was performed to identify the toxoplasma antigens from the mice tissue chronically infected with Toxoplasma gondii Beverley strain by peroxidase-antiperoxidaae(PAP) method. The 21 mice divided two group, 3 control group and 18 cortisone-treated group. In cortisone-treated group, the mice were injected with 0.5mg of cortisone acetate twice a week for 6 weeks subcutaneously and 3 mice were sacrified at each week. The brain, lung heart, liver, spleen, kidney, small intestine and skeletal muscle of mice were removed immediately after death. The tissues were fixed with 10% buffered neutral formalin, and then PAP method and H-E staining were done.
The results were obtained as follows :
1. The cysts were found in the brain entirely, in heart at 4 weeks, and in skeletal muscle at 5 weeks. The number and size of cysts in the brain were increased in proportion to total close of cortisone acetate in mice. Tachyzoites showed at 4.5 anct 6 week in brain.
2. The brain, spleen and skeletal muscle showed highly positive immunoreactivity. The liver, heart and small intestine were moderately positive and, lung and kidney showed weekly positive immunoreactivity by PAP method.
3. There was a chronic inflammatory responses in the brain, liver and heart, but the remaining organs were in almost normal appearance. And tachyzoites were found in the brain, associated with severe inflam matory changes.

목차 (Table of Contents)

• 목차
• I. 서론 = 1
• II. 실험재료 및 방법 = 1
• III. 실험성적 = 2
• IV. 고찰 = 3
• V. 결론 = 4
• 참고문헌 = 4
• Abstract = 6