DNA 메틸화는 유전체의 무결성의 유지 및 유전자 발현 조절과 같은 박테리아의 다양한 과정에 관여한다. Alphaproteobacteria 종에서 보존된 DNA 메틸 전이 효소인 CcrM은 S-아데노실 메티오닌을 공동...
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https://www.riss.kr/link?id=A106282293
김정희 (부경대학교 기초교양교육원) ; 오현명 (부경대학교) ; Kim, Junghee ; Oh, Hyun-Myung
2019
English
KCI등재,SCOPUS
학술저널
103-111(9쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
DNA 메틸화는 유전체의 무결성의 유지 및 유전자 발현 조절과 같은 박테리아의 다양한 과정에 관여한다. Alphaproteobacteria 종에서 보존된 DNA 메틸 전이 효소인 CcrM은 S-아데노실 메티오닌을 공동...
DNA 메틸화는 유전체의 무결성의 유지 및 유전자 발현 조절과 같은 박테리아의 다양한 과정에 관여한다. Alphaproteobacteria 종에서 보존된 DNA 메틸 전이 효소인 CcrM은 S-아데노실 메티오닌을 공동 기질로 사용하여 $N^6$-아데닌 또는 $N^4$-시토신의 메틸 전이 효소 활성을 갖는다. Celeribacter marinus IMCC 12053는 해양 환경에서 분리된 알파프로테오박테리아로서 GpC 시토신의 외향고리 아민의 메틸기를 대체하여 $N^4$-메틸 시토신을 생산한다. 단일 분자 실시간 서열 분석법(SMRT)을 사용하여, C. marinus IMCC12053의 메틸화 패턴을 Gibbs Motif Sampler 프로그램을 사용하여 확인하였다. 5'-GANTC-3'의 $N^6$-메틸 아데노신과 5'-GpC-3'의 $N^4$-메틸 시토신을 확인하였다. 발현된 DNA 메틸전이 효소는 계통 발생 분석법을 사용하여 선택하여 pQE30 벡터에 클로닝 후 $dam^-/dcm^-$ 대장균을 사용하여 클로닝된 DNA 메틸라아제의 메틸화 활성을 확인하였다. 메틸화 효소를 코딩하는 게놈 DNA 및 플라스미드를 추출하고 메틸화에 민감한 제한 효소로 절단하여 메틸화 활성을 확인하였다. 염색체와 메틸라아제를 코드하는 플라스미드를 메틸화시켰을 때에 제한 효소 사이트가 보호되는 것으로 관찰되었다. 본 연구에서는 분자 생물학 및 후성유전학을 위한 새로운 유형의 GpC 메틸화 효소의 잠재적 활용을 위한 외향고리 DNA 메틸라제의 특성을 확인하였다.
다국어 초록 (Multilingual Abstract)
DNA methylation is involved in diverse processes in bacteria, including maintenance of genome integrity and regulation of gene expression. CcrM, the DNA methyltransferase conserved in Alphaproteobacterial species, carries out $N^6$-adenine or $N^4$-cy...
DNA methylation is involved in diverse processes in bacteria, including maintenance of genome integrity and regulation of gene expression. CcrM, the DNA methyltransferase conserved in Alphaproteobacterial species, carries out $N^6$-adenine or $N^4$-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Celeribacter marinus IMCC12053 from the Alphaproteobacterial group was isolated from a marine environment. Single molecule real-time sequencing method (SMRT) was used to detect the methylation patterns of C. marinus IMCC12053. Gibbs motif sampler program was used to observe the conversion of adenosine of 5'-GANTC-3' to $N^6$-methyladenosine and conversion of $N^4$-cytosine of 5'-GpC-3' to $N^4$-methylcytosine. Exocyclic DNA methyltransferase from the genome of strain IMCC12053 was chosen using phylogenetic analysis and $N^4$-cytosine methyltransferase was cloned. IPTG inducer was used to confirm the methylation activity of DNA methylase, and cloned into a pQE30 vector using dam-/dcm- E. coli as the expression host. The genomic DNA and the plasmid carrying methylase-encoding sequences were extracted and cleaved with restriction enzymes that were sensitive to methylation, to confirm the methylation activity. These methylases protected the restriction enzyme site once IPTG-induced methylases methylated the chromosome and plasmid, harboring the DNA methylase. In this study, cloned exocyclic DNA methylases were investigated for potential use as a novel type of GpC methylase for molecular biology and epigenetics.
참고문헌 (Reference)
1 Loenen WA, "The other face of restriction : modification-dependent enzymes" 42 : 56-69, 2014
2 Gonzalez D, "The functions of DNA methylation by CcrM in Caulobacter crescentus : a global approach" 42 : 3720-3735, 2014
3 Eid J, "Real-time DNA sequencing from single polymerase molecules" 323 : 133-138, 2009
4 Roberts RJ, "REBASE—a database for DNA restriction and modification : enzymes, genes and genomes" 43 : D298-D299, 2015
5 Stamatakis A, "RAxML-VI-HPC : maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models" 22 : 2688-2690, 2006
6 Jeltsch A, "On the substrate specificity of DNA methyltransferases. adenine-N 6DNA methyltransferases also modify cytosine residues at position N 4" 274 : 19538-19544, 1999
7 Sohn JH, "Novosphingobium pentaromativorans sp. nov., a high-molecularmass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment" 54 : 1483-1487, 2004
8 Vincze T, "NEBcutter : a program to cleave DNA with restriction enzymes" 31 : 3688-3691, 2003
9 Ronquist F, "MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space" 61 : 539-542, 2012
10 Jurkowska RZ, "Mechanisms and biological roles of DNA methyltransferases and DNA methylation : From past achievements to future challenges" 945 : 1-17, 2016
1 Loenen WA, "The other face of restriction : modification-dependent enzymes" 42 : 56-69, 2014
2 Gonzalez D, "The functions of DNA methylation by CcrM in Caulobacter crescentus : a global approach" 42 : 3720-3735, 2014
3 Eid J, "Real-time DNA sequencing from single polymerase molecules" 323 : 133-138, 2009
4 Roberts RJ, "REBASE—a database for DNA restriction and modification : enzymes, genes and genomes" 43 : D298-D299, 2015
5 Stamatakis A, "RAxML-VI-HPC : maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models" 22 : 2688-2690, 2006
6 Jeltsch A, "On the substrate specificity of DNA methyltransferases. adenine-N 6DNA methyltransferases also modify cytosine residues at position N 4" 274 : 19538-19544, 1999
7 Sohn JH, "Novosphingobium pentaromativorans sp. nov., a high-molecularmass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment" 54 : 1483-1487, 2004
8 Vincze T, "NEBcutter : a program to cleave DNA with restriction enzymes" 31 : 3688-3691, 2003
9 Ronquist F, "MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space" 61 : 539-542, 2012
10 Jurkowska RZ, "Mechanisms and biological roles of DNA methyltransferases and DNA methylation : From past achievements to future challenges" 945 : 1-17, 2016
11 Kumar S, "MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets" 33 : 1870-1874, 2016
12 Maier JA, "Investigation of the C-terminal domain of the bacterial DNA-(adenine N 6)-methyltransferase CcrM" 119 : 60-67, 2015
13 Pérez A, "Impact of methylation on the physical properties of DNA" 102 : 2140-2148, 2012
14 Kozdon JB, "Global methylation state at base-pair resolution of the Caulobacter genome throughout the cell cycle" 110 : E4658-, 2013
15 Luo YR, "Genome sequence of benzo(a)pyrene-degrading bacterium Novosphingobium pentaromativorans US6-1" 194 : 907-, 2012
16 van der Wijst MGP, "Experimental mitochondria-targeted DNA methylation identifies GpC methylation, not CpG methylation, as potential regulator of mitochondrial gene expression" 7 : 177-, 2017
17 Herring JL, "Enzymatic methylation of DNA in cultured human cells studied by stable isotope incorporation and mass spectrometry" 22 : 1060-1068, 2009
18 Li Y, "Development of fluorescent methods for DNA methyltransferase assay" 5 : 012002-, 2017
19 Lawrence CE, "Detecting subtle sequence signals : a Gibbs sampling strategy for multiple alignment" 262 : 208-214, 1993
20 Adhikari S, "DNA methyltransferases and epigenetic regulation in bacteria" 40 : 575-591, 2016
21 Mohapatra SS, "DNA methylation in Caulobacter and other Alphaproteobacteria during cell cycle progression" 22 : 528-535, 2014
22 Harrison A, "DNA methylation : A timeline of methods and applications" 2 : 74-, 2011
23 Jang HS, "CpG and Non-CpG methylation in epigenetic gene regulation and brain function" 8 : 148-, 2017
24 Choi DH, "Complete genome sequence of Novosphingobium pentaromativorans US6-1T" 10 : 107-, 2015
25 Yang JA, "Complete genome sequence of Flavobacteriales bacterium strain UJ101 isolated from a xanthid crab" 5 : e01551-16-, 2017
26 Yang JA, "Complete genome sequence of Celeribacter marinus IMCC12053T, the host strain of marine bacteriophage P12053L" 26 : 5-7, 2016
27 Kang I, "Complete genome sequence of Celeribacter bacteriophage P12053L" 86 : 8339-8340, 2012
28 Eberhard J, "Cloning, sequence analysis and heterologous expression of the DNA adenine-(N 6)methyltransferase from the human pathogen Actinobacillus actinomycetemcomitans" 195 : 223-229, 2001
29 Renbaum P, "Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M. SssI)" 18 : 1145-1152, 1990
30 Xu M, "Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC" 26 : 3961-3966, 1998
31 Baek K, "Celeribacter marinus sp. nov., isolated from coastal seawater" 64 : 1323-1327, 2014
32 Marchler-Bauer A, "CDD : NCBI's conserved domain database" 43 : D222-D226, 2015
33 Eddy SR, "Accelerated profile HMM searches" 7 : e1002195-, 2011
분열효모에서 Thp1/PCID2의 이종상동체인 SPAC1B3.08이 mRNA 방출에 미치는 영향
Myxococcus stipitatus의 자실체 형성을 위한 배지 조성
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2013-12-02 | 학술지명변경 | 외국어명 : The Korean Journal of Microbiology -> Korean Journal of Microbiology | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2008-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2006-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2001-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
1998-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.21 | 0.21 | 0.21 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.26 | 0.24 | 0.48 | 0.02 |