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      KCI등재후보

      Human Embryonic Stem Cell-derived Neuroectodermal Spheres Revealing Neural Precursor Cell Properties = 인간 배아줄기세포 유래 신경전구세포의 특성 분석

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      https://www.riss.kr/link?id=A100234375

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      국문 초록 (Abstract)

      만능성 인간 배아줄기세포로부터 확립된 신경줄기세포 또는 신경전구세포는 퇴행성 신경질환 세포치료제로 이용될 수 있는 다양한 종류의 신경세포로 분화 유도될 수 있다. 하지만, 인간 배아줄기세포로부터 신경세포를 생산하기 위한 기술은 아직 많은 장애를 가지고 있다. 인간 배아줄기세포 유래 신경전구세포에서 특징적으로 나타나는 신경관 유사로제트에 대한 이해는 인간 배아줄기세포 신경 분화의 효율을 높이는데 유용한 정보를 제공할 것으로 사료된다. 일반적으로 신경로제트
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      만능성 인간 배아줄기세포로부터 확립된 신경줄기세포 또는 신경전구세포는 퇴행성 신경질환 세포치료제로 이용될 수 있는 다양한 종류의 신경세포로 분화 유도될 수 있다. 하지만, 인간 ...

      만능성 인간 배아줄기세포로부터 확립된 신경줄기세포 또는 신경전구세포는 퇴행성 신경질환 세포치료제로 이용될 수 있는 다양한 종류의 신경세포로 분화 유도될 수 있다. 하지만, 인간 배아줄기세포로부터 신경세포를 생산하기 위한 기술은 아직 많은 장애를 가지고 있다. 인간 배아줄기세포 유래 신경전구세포에서 특징적으로 나타나는 신경관 유사로제트에 대한 이해는 인간 배아줄기세포 신경 분화의 효율을 높이는데 유용한 정보를 제공할 것으로 사료된다. 일반적으로 신경로제트

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      다국어 초록 (Multilingual Abstract)

      Neural stem/precursor derived from pluripotent human embryonic stem cells (hESCs) has considerable therapeutic potential due to their ability to generate various neural cells which can be used in cell-replacement therapies for neurodegenerative diseases. However, production of neural cells from hESCs remains technically very difficult. Understanding neural-tube like rosette characteristic neural precursor cells from hESCs may provide useful information to increase the efficiency of hESC neural differentiation. Generally, neural rosettes were derived from differentiating hEBs in attached culture system, however this is time-consuming and complicated. Here, we examined if neural rosettes could be formed in suspension culture system by bypassing attachment requirement. First, we tested whether the size of hESC clumps affected the formation of human embryonic bodies (hEBs) and neural differentiation. We confirmed that hEBs derived from square sized hESC clumps were effectively differentiated into neural lineage than those of the other sizes. To induce the rosette formation, regular size hEBs were derived by incubation of hESC clumps() in EB medium for 1 wk in a suspended condition on low attachment culture dish and further incubated for additional wks in neuroectodermal sphere(NES)-culture medium. We observed the neural tube-like rosette structure from hEBs after days of differentiation. Their identity as a neural precursor cells was assessed by measuring their expressions of neural precursor markers(Vimentin, Nestin, MSI1, MSI2, Prominin-1, Pax6, Sox1, N-cadherin, Otx2, and Tuj1) by RT-PCR and immunofluorescence staining. We also confirmed that neural rosettes could be terminally differentiated into mature neural cell types by additional incubation for wks with NES medium without growth factors. Neuronal(Tuj1, MAP2, GABA) and glial( and GFAP) markers were highly expressed after and 4 wks of incubation, respectively. Expression of oligodendrocyte markers O1 and CNPase was significantly increased after wks of incubation. Our results demonstrate that rosette forming neural precursor cells could be successfully derived from suspension culture system and that will not only help us understand the neural differentiation process of hESCs but also simplify the derivation process of neural precursors from hESCs.
      번역하기

      Neural stem/precursor derived from pluripotent human embryonic stem cells (hESCs) has considerable therapeutic potential due to their ability to generate various neural cells which can be used in cell-replacement therapies for neurodegenerative diseas...

      Neural stem/precursor derived from pluripotent human embryonic stem cells (hESCs) has considerable therapeutic potential due to their ability to generate various neural cells which can be used in cell-replacement therapies for neurodegenerative diseases. However, production of neural cells from hESCs remains technically very difficult. Understanding neural-tube like rosette characteristic neural precursor cells from hESCs may provide useful information to increase the efficiency of hESC neural differentiation. Generally, neural rosettes were derived from differentiating hEBs in attached culture system, however this is time-consuming and complicated. Here, we examined if neural rosettes could be formed in suspension culture system by bypassing attachment requirement. First, we tested whether the size of hESC clumps affected the formation of human embryonic bodies (hEBs) and neural differentiation. We confirmed that hEBs derived from square sized hESC clumps were effectively differentiated into neural lineage than those of the other sizes. To induce the rosette formation, regular size hEBs were derived by incubation of hESC clumps() in EB medium for 1 wk in a suspended condition on low attachment culture dish and further incubated for additional wks in neuroectodermal sphere(NES)-culture medium. We observed the neural tube-like rosette structure from hEBs after days of differentiation. Their identity as a neural precursor cells was assessed by measuring their expressions of neural precursor markers(Vimentin, Nestin, MSI1, MSI2, Prominin-1, Pax6, Sox1, N-cadherin, Otx2, and Tuj1) by RT-PCR and immunofluorescence staining. We also confirmed that neural rosettes could be terminally differentiated into mature neural cell types by additional incubation for wks with NES medium without growth factors. Neuronal(Tuj1, MAP2, GABA) and glial( and GFAP) markers were highly expressed after and 4 wks of incubation, respectively. Expression of oligodendrocyte markers O1 and CNPase was significantly increased after wks of incubation. Our results demonstrate that rosette forming neural precursor cells could be successfully derived from suspension culture system and that will not only help us understand the neural differentiation process of hESCs but also simplify the derivation process of neural precursors from hESCs.

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      참고문헌 (Reference)

      1 Odorico JS, "Multilineage differentiation from human embryonic stem cell lines" 19 : 193-204, 2001

      2 Kim MS, "Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells" 7 : 513-515, 2007

      3 Assady S, "Insulin production by human embryonic stem cells" 50 : 1691-1697, 2001

      4 Zhang SC, "In vitro differentiation of transplantable neural precursors from human embryonic stem cells" 19 : 1129-1233, 2001

      5 Elkabetz Y, "Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage" 22 : 152-165, 2008

      6 Cho MS, "Highly efficient and large-scale generation of functional dopamine neurons from human embryonic stem cells" 105 : 3392-3397, 2008

      7 Carpenter MK, "Enrichment of neurons and neural precursors from human embryonic stem cells" 172 : 383-397, 2001

      8 Flax JD, "Engraftable human neural stem cells respond to developmental cues, replace neurons, and express foreign genes" 16 : 1033-1039, 1998

      9 Levenberg S, "Endothelial cells derived from human embryonic stem cells" 99 : 4391-4396, 2002

      10 Reubinoff BE, "Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro" 18 : 399-404, 2000

      1 Odorico JS, "Multilineage differentiation from human embryonic stem cell lines" 19 : 193-204, 2001

      2 Kim MS, "Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells" 7 : 513-515, 2007

      3 Assady S, "Insulin production by human embryonic stem cells" 50 : 1691-1697, 2001

      4 Zhang SC, "In vitro differentiation of transplantable neural precursors from human embryonic stem cells" 19 : 1129-1233, 2001

      5 Elkabetz Y, "Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage" 22 : 152-165, 2008

      6 Cho MS, "Highly efficient and large-scale generation of functional dopamine neurons from human embryonic stem cells" 105 : 3392-3397, 2008

      7 Carpenter MK, "Enrichment of neurons and neural precursors from human embryonic stem cells" 172 : 383-397, 2001

      8 Flax JD, "Engraftable human neural stem cells respond to developmental cues, replace neurons, and express foreign genes" 16 : 1033-1039, 1998

      9 Levenberg S, "Endothelial cells derived from human embryonic stem cells" 99 : 4391-4396, 2002

      10 Reubinoff BE, "Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro" 18 : 399-404, 2000

      11 Thomson JA, "Embryonic stem cell lines derived from human blastocysts" 282 : 1145-1147, 1998

      12 Itskovitz-Eldor J, "Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers" 6 : 88-95, 2000

      13 Perrier AL, "Derivation of midbrain dopamine neurons from human embryonic stem cells" 101 : 12543-12548, 2004

      14 Mummery C, "Cardiomyocyte differentiation of mouse and human embryonic stem cells" 200 : 233-242, 2002

      15 Joannides A, "Automated mechanical passaging: a novel and efficient method for human embryonic stem cell expansion" 24 : 230-235, 2006

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      학술지 이력

      학술지 이력
      연월일 이력구분 이력상세 등재구분
      2027 평가예정 재인증평가 신청대상 (재인증)
      2021-01-01 평가 등재학술지 유지 (재인증) KCI등재
      2018-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2015-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2011-01-01 평가 등재학술지 선정 (등재후보2차) KCI등재
      2010-01-01 평가 등재후보 1차 PASS (등재후보1차) KCI등재후보
      2009-01-01 평가 등재후보학술지 유지 (등재후보2차) KCI등재후보
      2008-01-01 평가 등재후보 1차 PASS (등재후보1차) KCI등재후보
      2007-01-01 평가 등재후보학술지 유지 (등재후보1차) KCI등재후보
      2005-01-01 평가 등재후보학술지 선정 (신규평가) KCI등재후보
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      학술지 인용정보

      학술지 인용정보
      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 0.4 0.4 0.32
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      0.27 0.25 0.394 0.13
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