콩 생육시기별 Lipoxygenase활성의 변화

국문 초록 (Abstract)

Lipoxygenase는 콩 비린내 유발의 주원인이 된다. 따라서 lipoxygenase 발현 양상을 알아보고자 콩 생육시기별로 재료를 채취하여 lipoxygenase activity를 측정한 바 그 결과는 다음과 같다. 1. V3 와 V5 시기에 콩 잎에서의 lipoxygenase activity는 유전자형에 상관없이 일정한 경향이 없었다. 2. R6 시기의 /pod wall에서도 유전자형에 따른 lipoxygenase activity의 일정한 경향은 없었으나 종자 lipoxygenase는 lipoxygenase 결핍계통의 activity가 일반품종에 비해 낮았으며 lipoxygenase 결핍계통간에도 isozyme 결핍정도에 따라 activity도 다르게 나타났다. 3. 종자를 종피, 배, 자엽으로 구분하여 각각의 lipoxygenase activity를 측정한 결과 종피에서는 유전자형에 따른 경향을 찾을 수 없었으나 배와 자엽에서는 lipoxygenase isozyme 결핍 정도에 따라 activity도 다르게 나타났다.

다국어 초록 (Multilingual Abstract)

Lipoxygenase is involved in the formation of certain undesirable flavors of soybean products. Three isozymes(L-1, L-2 and L-3) of lipoxygenase have been identified in soybean seeds, and the three types of mutants lacking L-1, L-2 and L-3, respectively, were detected in the 1980's. In this paper, lipoxygenase activity was measured to investigate the response of lipoxygenase in organs and tissues during soybean development. There was no tendency according to genotypes between lipoxygenase lacking mutants and normal soybeans in lipoxygenase activity of leaf at V3 and V5 stage. Likewise, pod wall lipoxygenase was no difference among genotypes tested at R6 stage. Seed coat lipoxygenase activity was similar among the lipoxygenase lacking mutants, while normal soybean was lower as compared with that of the lipoxygenase lacking mutants. Embryo and cotyledon lipoxygenase activity in the lipoxygenase lacking mutants was much lower than that of normal soybean, also there was large difference among lipoxygenase lacking mutants. Thus, the lipoxygenase null mutant showed very weak value although the lacking L-3 mutant had a large effect on developing embryo lipoxygenase activity. It was suggested that soybean lipoxygenase isozymes expressed in embryo may be different from those expressed in the pod wall and leaf tissues.