鷄胎器官性長에 미치는 Mitoycin C의 影響에 關한 硏究 = Effects of Mitomycin C on the Growth of Chick Embryo

다국어 초록 (Multilingual Abstract)

Administration of Mitomycin C into various species of animals has widely been reported since last decade, however, none of them were utilized a growth formula proposed by Zimmerman and Huxley. In this paper we attempt to analyze the specific growth rate in general and organ relative growth rate using the chick embryo under the influence of Mitomycin C which is well known as an antibacterial, antiviral and antitumor by the mechanism of the alkylation. Congenital malformation of chick embryo were not considered.
On the sixth day incubation, 8㎍ and 16㎍ of Mitomycin C were injected into the chorioallantoic and the egg was broken for weighing the body weight and individual organs such as brain, heart and lung on the day of 12th, 14th, 16th and 18th.
The effects of the growth were analyzed by the analysis of variance method. The parameters included in growth quantity were obtained by the parameters included in growth quantity were obtained by the mathod of the last square using the measured weight, the formulas applied to analysis growth quantity and relative growth were : y=a+bt+ct^2, y=bx^2, and the results this obtained were summarized as follows :
When injected with Mitomycin C, the chick embryos were observed with remarkable growth inhibition in all groups of the body, brain, lung and 14th groups of heart (p<0.005) and 12th, 16th, 18th groups of heart(p<0.05). Growth rate was increased with the development in the 8㎍ injected groups of the body, lung and 16㎍ injected groups of the lung, on the other hand, it was decreased in the both injected group of the brain and heart.
The specific growth rate of all groups showed tendency of decrease in 8㎍ and 16㎍ Injected groups.
The coefficient of relative growth of injected groups to control groups in the various organs showed positive allometry. The growth center of the growth gradient of all injected groups to control groups were on the eighteenth day.