Analysis of Hybridization in Korean Ecotypes of Poa pratensis L. Using RAPD and SCAR Markers = RAPD와 SCAR 표지를 이용한 자생 켄터키블루그래스 생태형의 교배 분석

국문 초록 (Abstract)

한국에는 켄터키블루그래스(왕포아풀, Poapratensis L.)의 다양한 생태형이 서식하고 있으며, 이들 중 일부는 잔디적 특성이 우수할 뿐만 아니라 높은 단위생식성을 나타내고 있다. 본 연구는 계통 특이적 DNA 표지를 개발하고 이를 이용하여 생태형들의 교배 효율을 분석하기 위하여 수행되었다. 생태형들과 육성종(Midnight, Unique, Challenger, Award, Blacksburg)들 간에 115조합의 교배를 수행하여 8,612 개체의 후대 식물을 얻었으며, 이로부터 지면피복률이 뛰어나고 엽색이 뛰어난 ‘2-73’과 엽밀도와 봄 green-up이 우수한 ‘31-40’ 계통을 선발하였다. 생태형 27종과 육성종 5종에 대한 RAPD 분석에서 OPB 프라이머(#4, 5, 7, 8, 10, 18)를 이용하여 20개의 계통 특이적 표지를 얻었으며, 이들 염기서열을 분석하여 SCAR 표지 14개를 개발하고 이들 표지의 조합으로 16종의 계통들을 식별할 수 있게 되었다. 또한 국내 생태형을 종자친으로하고 육성종을 화분친으로 교배하여 선발된 우수 계통 ‘2-73’과 ‘31-40’의 교배 여부를 교배 모본의 SCAR 표지를 통하여 확인할 수 있었다.

다국어 초록 (Multilingual Abstract)

Ecotypes of Poa pratensis that exhibit desirable traits as turfgrass such as fast proliferation for ground covering and environmental tolerances were collected in southern Korea. Like most typical varieties of Kentucky bluegrass, the ecotypes had varying levels of apomixis which resulted in low hybridization efficiency. Therefore, experiments were carried out to (1) develop genotype-specific RAPD and SCAR markers, and (2) to determine hybridization rates among the ecotypes and to aid the selection of desirable hybrids. Hybridization among the ecotypes and improved cultivars such as ‘Midnight’, ‘Unique’, ‘Challenger’, ‘Award’, and ‘Blacksburg’ were performed using an in vitro hybridization technique. From 115 crossing combinations among 47 ecotypes and improved cultivars, 8,612 progeny plants were obtained. Desirable progenies were identified in ‘2-73’ which was outstanding in ground covering from a seeded plug as well as its fine texture with darker blue leaf color, and ‘31-40’, which showed relatively early spring green-up. Random Amplified Polymorphic DNA (RAPD) profiles of 27 ecotypes and 5 improved cultivars were examined using OPB primers of 4, 5, 7, 8, 10 and 18, and twenty polymorphic markers were obtained. Sequences for six of the polymorphic RAPD bands were developed into Sequence Characterized Amplified Region (SCAR) markers. The SCAR markers were useful in determining crossing rates between the ecotypes and the improved cultivars used as pollen parents. They were also used to confirm the hybridization of selected progenies like ‘2-73’ (‘P77’ × ‘Midnight’) by the presence of a SCAR marker of the pollen parent.

목차 (Table of Contents)

• Abstract
• Introduction
• Materials and Methods
• Results and Discussion
• Literature Cited
• 초록