Chondrogenesis of Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood

다국어 초록 (Multilingual Abstract)

In the current study, the mesenchymal stem cells (MSCs) isolated and propagated from the human umbilical
cord blood (UCB) were tested for their capabilities of differentiation into chondrocytes in vitro. The mesenchymal
progenitor cells (MPCs) collected from UCB were cultured in a low glucose DMEM medium with 10% FBS, Lglutamine
and antibiotics. The human MSC colonies were positively stained by PAS reaction. When the
immunophenotypes of surface antigens on the MSCs were analyzed by fluorescence-activated cell sorter (FACS)
analysis, these cells expressed positively MSC-related antigens of CD 29, CD44, CD 90 and CD105, whereas they
did not express antigens of CD14, CD31, CD34, CD45, CD133 and HLA-DR. Following induction these MSCs into
chondrocytes in the chondrogenic differentiation medium for 3 weeks or more, the cells were stained positively with
safranin O. We clearly confirmed that human MSCs were successfully differentiated into chondrocytes by RT-PCR
and immunofluorescent stain of type-II collagen protein. These data also indicate that the isolation, proliferation and
differentiation of the hUCB-derived MSCs in vitro can be used for elucidating the mechanisms involved in
chondrogenesis. Moreover this differentiation technique can be applied to developing cell-based tissue regeneration
or repair damaged tissues

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