As a preliminary investigation to obtain useful auto- and allopolyploids a ten year old tetraploid of Pinus densiflora induced from the colchicine treated seed was observed on the cytological, morphological and physioa logical characters in the presen...
As a preliminary investigation to obtain useful auto- and allopolyploids a ten year old tetraploid of Pinus densiflora induced from the colchicine treated seed was observed on the cytological, morphological and physioa logical characters in the present study and the results can be summarized as follows.
1. The number of chromosome at the base of needle was 48 so that it was recognized as a tetraploid.
2. The needles were thicker and the number of them on an individual was less than the 2n plant. The needle combined to a single was appeared 2%.
3. No difference was found in the number of stomata rows on the central part of needle between the tetraploid and 2n plant, however, guard cells of tetraploid increased 36% in the long diameter compared with 2n plant.
4. Microsporangiate storbile developed normally showing the same size with 2n plant while the size of pollen grain increased about 20% larger than 2n plant.
5. Germination percentage in vitro increased until 17 hours but decreased after 26 hours compared with 2n plant. The longest length of pollen tube during the germination period appeared in the tetraploid pollen.