1P-178 Enzyme-Mediated Reaction for the Development of Antimicrobial Surfaces through Graphene Oxide Immobilization = 1P-178 Enzyme-Mediated Reaction for the Development of Antimicrobial Surfaces through Graphene Oxide Immobilization

다국어 초록 (Multilingual Abstract)

Nowadays, to overcome the implant-related infection issue, many researches have been done to develop the antimicrobial surfaces, including the immobilization of antibacterial agents. Herein, graphene oxide (GO), a popular antibacterial agent with long-term and excellent activity, was immobilized on titanium dioxide (TiO₂) substrates via tyrosinase-catalyzed reaction. GO was oxidized from graphene, following by the conjugation of the phenol groups using EDC/NHS chemistry, making GO capable to be oxidized by tyrosinase. These phenol moieties were oxidized into o-quinones by tyrosinase, can easily attach to TiO₂. The GO immobilized surface show the antibacterial effect against both E.coli and S.aureus. Besides, they also show the biocompatibility with human dermal fibroblasts (hDFBs). This study presents a facile and rapid approach for enhancing antibacterial activity of TiO₂.