Chinese hamster 난소세포에서 DNA - 단백질 교차결합과 glutathione S - 전이효소의 발현에 대한 돌연변이원에 의해 유도된 교차적응반응 = Cross - Adaptive Response Induced by Mutagens to DNA - Protein Crosslink and Expression of Glutathione S - Transferase in Chinese Hamster Ovary Cells

다국어 초록 (Multilingual Abstract)

The present study has been undertaken to elucidate whether glutathione S-transferase (GST) is related to cross-adaptive response to DNA-protein crosslink (DPC) induced by ethyl methanesulfonate (EMS) or ultraviolet radiation (UV)-C in Chinese hamster ovary (CHO) cells. DPC was determined by SDS-K^+ precipitation method, and GST was measured by western blot analysis. 2,4-Dinitrophenol (DNP) that depletes cellular ATP and cycloheximide (CHM) that inhibits protein synthesis were used to find whether DNP and CHM affected the expression level of GST and cross-adaptive response to EMS or UV. There is a cross-adaptive response to DPC in cells pretreated with EMS or UV and subsequently treated with UV or EMS, respectively. In cells pretreated with 2 mM EMS and subsequently treated with 5J/㎡ UV, DNP and CHM did not affect cross-adaptive response to DPC and GST. And also, in cells pretreated with 1J/㎡ UV and subsequently treated with 8 mM EMS, CHM did not affect crass-adaptive response to DPC and the expression level of GST. But DNP increased the amount of DPC, and decreased the expression level of GST in UV-pretreated cells. These results suggest that GST are involved in the induction of cross-adaptive response to DPC in CHO cells.