Mycotoxins Containing Diet Affects Oocyte Quality in Mouse

다국어 초록 (Multilingual Abstract)

Background: Mycotoxins which mainly consist of Aflatoxin (AF), Zearalenone (ZEN)
and Deoxynivalenol (DON) are commonly found in many food commodities, each component
has been shown to cause organ toxicity and oxidative stress in several species.
Our previous study showed that mycotoxin-contaminated diet could cause oxidative
stress in liver, kidney, spleen. Recently we examined its effects on oocyte quality.
Materials and Methods: Mycotoxins-contaminated maize (AF 597μg/kg, ZEN 729μg/kg,
DON 3.1mg/kg maize) was incorporated into the diet at three different doses (0, 5 and
20%) to feed the mice for 4 weeks.
Results: Our results showed that the both the index of ovary and the number of
good GV oocytes decreased in the mycotoxin-treated mice. The oocytes from mycotoxin-
treated mouse displayed low developmental competence showing with lower GVBD
and polar body extrusion rate; the embryo developmental competence also showed the
similar pattern, most embryos could not develop to blastocyst stage. The cytoskeleton
component actin expression in both oocyte cortex and cytoplasm decreased, and the
expression of actin nucleation factor Profilin and mDia1 also decreased, indicating that
mycotoxin may affect oocyte quality through the effects on actin. Moreover, a big proportion
of oocytes with mycotoxin contaminated diet treatment showed disrupted cortical
granule free domain, spindle morphology and mitochondria distribution, further confirmed
the oocyte quality declination. We also used the in vitro model to confirm this, we cultured the oocytes in the medium with Zearalenone, a key component of mycotoxins,
and the results were similar with the in vivo model.
Conclusion: Our data indicated that the mycotoxins were toxic to mouse reproductive
system and induced the oocyte quality declination.