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      Novel Streptavidin-Functionalized Silicon Nanowire Arrays for CD4<sup>+</sup> T Lymphocyte Separation

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      https://www.riss.kr/link?id=A107622441

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      <P>Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4<SUP>+</SUP> T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4<SUP>+</SUP> T lymphocytes. Fabricated SiNW arrays easily separated the CD4<SUP>+</SUP> T lymphocytes from the mouse whole splenocytes with over ∼88% purity and demonstrated tight attachment to CD4<SUP>+</SUP> T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.</P><P><B>Graphic Abstract</B>
      <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2010/nalefd.2010.10.issue-8/nl100942p/production/images/medium/nl-2010-00942p_0007.gif'></P>
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      <P>Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4<SUP>+</SUP> T lymphocytes from the heterogeneo...

      <P>Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4<SUP>+</SUP> T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4<SUP>+</SUP> T lymphocytes. Fabricated SiNW arrays easily separated the CD4<SUP>+</SUP> T lymphocytes from the mouse whole splenocytes with over ∼88% purity and demonstrated tight attachment to CD4<SUP>+</SUP> T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.</P><P><B>Graphic Abstract</B>
      <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2010/nalefd.2010.10.issue-8/nl100942p/production/images/medium/nl-2010-00942p_0007.gif'></P>

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