Tobamovirus Coat Protein CPCg Induces an HR-like Response in Sensitive Tobacco Plants

다국어 초록 (Multilingual Abstract)

When inoculated into sensitive tobacco Xanthi-nn plants, the crucifer and garlic-infecting Tobacco mosaic virus (TMV-Cg) induces local necrotic lesions that
resemble those seen in the hypersensitive response (HR) of resistant tobacco plants. However, unlike these, tobacco Xanthi-nn plants do not become resistant to
infection and the virus spreads systemically causing a severe disease characterized by necrotic lesions throughout the plant.
To identify the viral protein that elicits
this necrotic response, we used a set of hybrid viruses constructed by combination of TMV-Cg and the tobacco mosaic virus strain U1 (TMV-U1). In this study we present evidence that the coat protein of TMV-Cg (CPCg) is the elicitor of the necrotic response in tobacco
Xanthi-nn plants. Local and systemic necrotic lesions induced by TMV-Cg and by the hybrid U1-CPCg -that carries CPCg in a TMV-U1 context- are characterized by cell death and by the presence of autoflorescent phenolic compounds and H2O2, just like
the HR lesions. In addition, defense-related genes and detoxifying genes are induced in tobacco Xanthi-nn plants after TMV-Cg and U1-CPCg inoculation.
We postulate that in our system, CPCg is recognized by sensitive tobacco plants that mount an incomplete defense
response. We call this an HR-like since it is not enough to induce plant resistance.

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