생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가 = Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract

다국어 초록 (Multilingual Abstract)

Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List.
In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17 ~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 ㎎/L sucrose, and 2 ㎎/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content.
The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies

국문 초록 (Abstract)

황근(Hibiscus hamabo Sieb. et Zucc., 노랑무궁화)은 주로제주도 전역과 전남 남부지역에 자생하는 우리나라 유일의 야생 무궁화속의 낙엽성 반관목식물이다. 최근 무분별한 자생지파괴에 따른 개체수 감소로 환경부 지정 한국적색목록 내 멸종위기야생생물 2급으로 지정되어 있다. 선행연구에서는 황근의종자발아, 생태 및 유전적 특성, 내염성 등이 보고되었다. 따라서 본 연구에서는 생물반응기를 이용하여 황근 부정근의 대량증식, 항산화 및 미백효과를 조사하여 기능성 원료 활용 평가를 실시하였다. 황근 종자 및 식물체는 사전 환경부 채취 허가를 받은 제주시 구좌읍 관내지역에서 채집하였으며, 표면살균 후 MS 등 배지 조성을 조절하여 기내 세포주로 도입하였다. 그 결과, 종자의 전반적인 반응율이 지상부 신초 발생 및 지하부 뿌리 발근 측면에서 51.17∼51.83%로 가장 효율적인 것으로 나타났다.
5,000 mL 생물반응기에서 황근 부정근의 증식에 유리한 배지조건의 경우, 1/2 MS (Murashige and Skoog, 1962), 30 g/L 자당(sucrose), 2 ㎎/L IBA (indole-3-butyric acid)에서 8주 동안 배양한 것임을 확인하였다. 또한 총페놀 화합물 함량, 총플라보노이드 함량, DPPH 자유 라디칼 소거능 및 멜라닌 함량 분석을 통해 바이오매스 및 2차대사산물 축적의 연관관계를 비교하였다. 본 연구를 통해 황근 부정근의 대량증식, 항산화 및 미백 효능은 고부가가치 향장품 산업의 고품질 소재개발과 연계하여 추가적인 생리활성물질 연구의 가능성을 시사한다.

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