Inhibitory Effects of (-) Epigallocatechin Gallate and Quercetin on High Glucose-induced Endothelial Cytotoxicity

다국어 초록 (Multilingual Abstract)

Functional damage to microvascular endothelial cells by hyperglycemia is thought to be one of the critical risk factors in the impaired wound healing seen with diabetes mellitus. It is also thought that oxidative stress plays a significant role in this endothelial cell dysfunction. The present study examined the differential effects of flavonoids on endothelial cell dysfunction under high glucose condition. Human endothelial cells exposed to 30 m㏖/ℓ glucose for 7 d were pre-treated with various flavonoids and pulse-treated with 0.2 m㏖/ℓ H₂O₂ for 30 min. High glucose markedly decreased cell viability with elevated oxidant generation and nuclear condensation, H₂O₂ insult exacerbated endothelial cytotoxicity due to chronic exposure to high glucose. (-)Epigallocatechin gallate and quercetin improved glucose-induced cell damage with the disappearance of apoptotic bodies, whereas apigenin intensified the glucose cytotoxicity. In addition, cell viability data revealed that these flavonoids of (-)epigallocatechin gallate and quercetin substantially attenuated both high glucose- and H₂O₂- induced dual endothelial damage. 'These results suggest that (- )epigallocatechin gallate and quercetin may be beneficial agents for improving endothelial cell dysfunction induced by high glucose and may prevent or reduce the development of diabetic vascular complications.

목차 (Table of Contents)

• INTRODUCTION
• MATERIALS AND METHODS
• RESULTS AND DISCUSSION
• Literature cited